--Highlights prepGEM(TM) Tissue Kit’s Rapid, Robust and Reliable DNA Extraction That Generates DNA Templates Outperforming Other Commercial Kits--
-- Samples Prepared from Mouse Tails Using prepGEM(TM) Yielded Over 34 Times the Amount of DNA Produced by Two Commercial Rapid Extraction Kits --
SOLANA BEACH, Calif. and HAMILTON, New Zealand, May 7 /PRNewswire/ -- ZyGEM Corp. Ltd., a provider of innovative enzyme-based products for DNA extraction and other life sciences applications, today announced the availability of a new application note documenting the performance advantages of its prepGEM(TM) kits compared to other rapid commercial kits for small animal genotyping. The note specifically evaluates ZyGEM’s prepGEM(TM) Tissue DNA extraction kits used in PCR assays for genotyping mice, a commonly-performed biomedical research application.
In the application note, ZyGEM researchers describe their experience extracting DNA and determining precise genotypes from mouse tail samples. Researchers performed extractions comparing ZyGEM’s prepGEM(TM) Tissue kit with two other commercially available kits with similar protocols. They then amplified a relevant segment of DNA using PCR reagents supplied in the kits and also with an external amplification system. The prepGEM(TM) Tissue extractions and amplifications yielded superior results based on both qualitative and quantitative PCR comparisons of the amplified DNA.
“This new application note focusing on mouse genotyping demonstrates that our prepGEM(TM) Tissue kits easily produce rapid, robust and reliable DNA extraction that outperformed the other kits tested,” said Paul Kinnon, CEO of ZyGEM. “Since small animal genotyping is a critical but often time-consuming aspect of biomedical research, we are pleased to make this comparative data available to our growing customer base.”
The results included in this application note show that in a PCR assay gel analysis, the prepGEM(TM) Tissue kit extractions yielded readily amplified DNA with no migration distortion or smearing, while the other two commercial kits yielded DNA with migration distortion or low band intensity, indicating a low yield of extracted DNA. The quantitative PCR analysis showed that samples prepared with prepGEM(TM) Tissue yielded over 34 times the amount of DNA produced by the higher-yielding of the two comparison kits.
The prepGEM(TM) Tissue kit is compatible with all PCR detection and amplification reagents, and the stability of the prepGEM(TM) reagents allows convenient storage at 4 degrees C, reducing preparation time and increasing ease of use.
ZyGEM application notes can be accessed at http://zygem.com/Products/Products-Pubs.html.
ZyGEM’s proprietary technology uses a thermophilic enzyme to extract DNA from samples using a single closed-tube system. ZyGEM’s approach significantly reduces the time and cost of DNA extractions while simplifying laboratory workflow, minimizing error, reducing the risk of contamination, ensuring sample integrity and facilitating extractions from trace samples. It is easily adaptable for use with virtually all off-the-shelf laboratory automation systems. The technology can be specifically formulated for use with a wide range of different sample types without the need for further purification. ZyGEM’s technology has been validated for use in highly regulated applications, including forensic analysis.
About ZyGEM’s DNA Extraction Kits
prepGEM(TM) kits have been formulated for high-throughput DNA extraction from animal tissue samples for life science research, animal testing and traceability, selective breeding and other animal health and agricultural applications. prepGEM(TM) kits enable high-throughput DNA extraction of 384 samples in less than 30 minutes. They contain reagents that have been optimized for downstream PCR-based applications including quantitative PCR, genotyping using STRs or SNPs, RFLP analyses, etc. prepGEM(TM) kits are proven on a range of animal samples including blood, micro-biopsies (ear tags), meat, rat tails, fish, fish tails and insects.
forensicGEM(TM) kits have been formulated for high-throughput DNA extraction from crime scene and other sample sources, providing PCR-ready, forensic grade DNA for 96 samples in just 20 minutes. The one-tube forensicGEM(TM) process is simple to use and easy to automate with current robotic platforms, making it ideal for database testing as well as case work samples. All reagents are rigorously quality controlled to test for efficacy and for the absence of human DNA. forensicGEM(TM) kits are validated for forensic analysis.
livestockGEM(TM) kits have been formulated for high-throughput DNA extraction from a range of animal sample types without the need for further purification, including ear punch microbiopsies, saliva and hair, as well as from blood and saliva DNA storage cards. Using ZyGEM’s technology, staff can prepare DNA samples up to three times faster than with existing products--the kits provide PCR-ready DNA for 96 samples in just 20 minutes with no hands-on attention required. livestockGEM(TM) kits are easy to use and to automate for high-throughput applications using off-the-shelf robotic systems.
For more information on ZyGEM’s families of DNA extraction kits, visit http://zygem.com/Products/Products-Ovw.html or email info@zygem.com
About ZyGEM Corporation
ZyGEM Corporation Limited is a rapidly growing biotechnology company with a range of innovative enzyme-based products and technologies based on the company’s exclusive collection of microorganisms from extreme environments. ZyGEM’s technology has significant competitive advantages over existing methods and has potential applications in a number of sizeable global markets. ZyGEM currently produces DNA extraction and detection products, molecular biology enzymes and individual enzymes and diagnostics, serving life sciences customers in basic research, forensics, clinical diagnostics and agriculture. ZyGEM has facilities in New Zealand and the U.S.A. For more information, visit www.ZyGEM.com
CONTACT: Paul Kinnon, President and CEO of ZyGEM Corp. Ltd.,
+1-858-720-8333; or Barbara Lindheim of GendeLLindheim BioCom Partners,
+1-212-918-4650
Web site: http://www.ZyGEM.com/
