ROCKFORD, Ill. (April 9, 2009) — Thermo Fisher Scientific, the world leader in serving science, announces the addition of two new high content screening (HCS) kits to its popular Thermo Scientific Cellomics HCS product line. These new products include the Phospho-mTOR Kit and the Mulitplex Mitosis-Apoptosis Kit. As with other Cellomics® HCS Reagents Kits, the new kits combine fluorescent probes, antibodies, reagents and optimized protocols to streamline the preparation of imaging samples for automated cell-based assays.
The Thermo Scientific Cellomics Phospho-mTOR Kit measures phosphorylation of mTOR, a kinase involved in the initiation of ribosome biogenesis and translation. The kit contains a monoclonal rabbit anti-phospho-mTOR antibody, a goat anti-rabbit secondary antibody conjugated to Thermo Scientific DyLight 549 Fluorophore and various other reagents and buffers required for immunofluorescence detection of mTOR in HCS assays.
The Thermo Scientific Cellomics Multiplex Mitosis-Apoptosis Kit enables the simultaneous quantification of nuclear DNA content, BrdU incorporation and active caspase 3 and p53 proteins. This kit allows for measurements of cell proliferation (cell number, DNA replication) and apoptosis using a fixed end-point assay based on immunofluorescence detection in cells grown on standard high-density microplates. DAPI is a DNA-binding dye used to determine the nuclear size and nuclear morphology as well as cell cycle phases by DNA content. The primary antibodies included in the kit are specific for their targets and have minimal cross-reactivity.
The Phospo-mTOR and Multiplex Mitosis-Apoptosis Kits are designed and validated with the Cellomics ArrayScan® HCS Reader and BioApplications Software, providing a total solution for high content screening. The kits are also fully compatible with other fluorescence-based HCS platforms and conventional fluorescence microscopy. Both kits come in two sizes, supplying sufficient reagents for 5 x 96 wells or 50 x 96 wells.
For more information on the Cellomics Phospho-mTOR Kit or Multiplex Mitosis-Apoptosis Kit, visit www.thermo.com/hcs or call +1-815-968-0747.
Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10.5 billion, we have approximately 34,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. The Thermo Scientific brand represents a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com.
