PENZBERG, Germany--(BUSINESS WIRE)--Quantitative real-time reverse-transcription PCR (qPCR) is a frequently used research tool in human glioma. So far, there has been no systematic approach to analysis of suitable reference genes for reliable gene expression analysis. In a recent study by Kreth et al. (1), 19 commonly used reference genes were tested for their expression stability in human astrocytoma WHO Grade II, astrocytoma WHO Grade III, and glioblastoma (WHO Grade IV) both alone and compared with normal brain. Equivalence tests for equal expression of candidate genes were applied. Genes showing differential expression were ruled out from further analyses. The authors used the RealTime ready Human Reference Gene Panel from Roche Applied Science (SIX: RO, ROG; OTCQX: RHHBY), which permits simultaneous expression analysis of the 19 reference genes. Real-time qPCR was performed in duplicate with the Roche LightCycler® 480 Instrument, 96-well plate. The researchers used the NormFinder software to determine expression stability of the remaining candidate genes.
