The convenient, ready-to-use Pierce® Peptide Retention Time Calibration Mixture contains 15 synthetic, heavy peptides mixed at an equimolar ratio to elute across the chromatographic gradient. It can be used with Thermo Scientific Pinpoint Software to predict peptide retention time from sequence alone, using hydrophobicity factors, or to predict peptide retention time between instrument platforms.
The Pierce Peptide Retention Time Calibration Mixture streamlines the transition from qualitative protein discovery results to the development of targeted mass spectrometry (MS) assays on Thermo Scientific Triple Quadrupole, Orbitrap and Exactive Instruments and all other mass spectrometers. It also saves time in peptide purification by increasing the prediction efficiency of peptide retention profiles. The mixture is useful in evaluating different reversed-phase column and gradient options, monitoring for autosampler and HPLC column performance characteristics and normalizing results between experiments and over time.
For more information on the Pierce Peptide Retention Time Calibration Mixture, please visit www.thermoscientific.com/pierce.
About Thermo Fisher Scientific
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