The Pierce® GTPase Enrichment Kit expands on the use of novel desthiobiotin-nucleotide probes that are also found in the new Thermo Scientific Pierce Kinase Enrichment Kits. The nucleotide is attached to desthiobiotin, a biotin analog developed by ActivX Biosciences. The nucleotide binds to the active site of the GTP-binding protein, and the desthiobiotin tag allows for capture and enrichment of the bound protein. The desthiobiotin tag binds less tightly to biotin-binding proteins, making the capture bond easily reversible by biotin displacement, low pH or heat. The isolated proteins then can be further studied by Western blot analysis or mass spectrometry.
This kit provides all labeling and enrichment reagents, including the ActivX GTP probe. The ActivX Probe is also available separately. For more details on the Pierce GTPase Enrichment Kit, please visit www.thermoscientific.com/pierce.
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