Researchers in the Department of Biosciences and Nutrition at the Karolinska Institute in Stockholm, Sweden, have been using Tecan’s Infinite® 200 microplate reader and NanoQuant Plate™ as one of the methods for the characterization of a newly isolated population of skin stem cells in mice (Lgr5+ cells).
Dr Maria Kasper, from the Karolinska Institute’s environmental toxicology group, explained how the system is helping the research: “The scarcity of Lgr5+ cells makes the extracted genetic material extremely valuable, so we need a method to achieve exact quantification of this RNA with minimal sample loss. Using our Infinite reader with the NanoQuant Plate ensures that only minimal material is required for reliable evaluation of our valuable RNA samples, and allows us to investigate more than 30 selected genes by qPCR from just 150 ng of isolated RNA.”
Maria’s colleague, Dr Viljar Jaks, continued: “Because the NanoQuant Plate is integrated into the Infinite microplate reader, it is very easy to use and the i-control™ software is intuitive. Having it in the laboratory has been very useful for our investigations.”
To find out more on Tecan’s Infinite 200 series microplate readers, visit www.tecan.com/infinite
To find out more on Tecan’s NanoQuant Plate, visit www.tecan.com/nanoquant
About Tecan
Tecan (www.tecan.com) is a leading global provider of laboratory instruments and solutions in biopharmaceuticals, forensics, and clinical diagnostics. The company specializes in the development, production and distribution of automation solutions for laboratories in the life sciences sector. With its subsidiary REMP (www.remp.com), Tecan is the market leader in automated laboratory storage and logistics systems. Its clients include pharmaceutical and biotechnology companies, university research departments and diagnostic laboratories. Founded in Switzerland in 1980, the company has manufacturing, research and development sites in both North America and Europe and maintains a sales and service network in 52 countries. In 2008, Tecan generated sales of CHF 396 million (USD 366.7 million; EUR 250.7 million). Registered shares of Tecan Group are traded on the SIX Swiss Exchange (TK: TECN/Reuters: TECZn.S/Swiss security number: 1210019).
