18 October 2011, Madison, WI USA: Promega Corporation announced today that it has launched GoTaq® Long PCR Master Mix, an optimized enzyme mixture for improved long-range PCR. The new kit enhances PCR yield, sensitivity and specificity and enables efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The GoTaq Long PCR Master Mix components have been engineered with hot start formulation to enable simple reaction set-up, and optimized to provide consistently efficient, accurate and robust performance of long DNA amplicons. The new master mix is ideal for cloning genes, mutational analysis and DNA sequencing.
The GoTaq Long PCR Master Mix contains Promega’s high performance GoTaq Hot Start Polymerase in a specially formulated mixture with a second proprietary thermal stable proofreading polymerase. The presence of a proofreading enzyme to repair DNA mismatches in the presence of a highly processive polymerase allows increased DNA elongation, resulting in longer DNA amplification.
GoTaq Long PCR Master Mix consists of a master mix containing the polymerase and proofreading enzyme mixture, dNTPs, and MgCl2. Also provided are control primers, human genomic control DNA, and nuclease-free water. Reaction set-up simply requires the user to add their DNA template, primers and nuclease-free water to the master mix.
For more information about GoTaq Long PCR Master Mix, please visit: http://www.promega.com/longrange
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Enquiries:
Katie Odgaard
Zyme Communications Ltd
Tel: +44 (0) 7787 502 947
Skype: kodgaard
Email: katie.odgaard@zymecommunications.com
www.zymecommunications.com
Penny Patterson
Director, Corporate Affairs
Promega Corporation
Phone: +1 (608) 274-4330
E-mail: penny.patterson@promega.com
www.promega.com
About Promega
Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. The company’s 2,000 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, molecular diagnostics and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA, with branches in 15 countries and over 50 global distributors. For more information about Promega, visit: www.promega.com