Spin-column Chromatrap ChIP Assay Used With Low-abundant Targets
Published: Jun 20, 2012
A recent study by the Reproductive Biology & Cancer Research group at the Institute of Life Science, Swansea University compared the Chromatrap performance to that of the magnetic bead based assay targeted at ER? and the less abundant ER? isoform using a Human endometrial epithelial cell line Ishikawa, grown in the presence of Estradiol and Tamoxifen. Chromatrap was found to be as sensitive in terms of % real signal binding of ER? on the XBP1 promoter as the bead based assay despite using a much lower chromatin input template and concentration (less than half) of ER? antibody. The performance of the Chromatrap ChIP kit was even more robust when used to detect binding of the less abundant ER??isoform on the XBP1 promoter. Full details are available in a newly published Application Note.
For a copy of the Application Note please visit http://www.chromatrap.com/technical-library/application-notes/ or contact Porvair Filtration Group on +44-1489-864330 / +1-804-550-1600 / email email@example.com.
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