ROCKFORD, Ill. (August 5, 2010) – Thermo Fisher Scientific, the world leader in serving science, today introduced the Thermo Scientific Pierce Glutathione Agarose family of products for cost-effective purification of GST-tagged proteins from cellular lysates. Pierce Glutathione Agarose consists of glutathione linked to agarose beads though a 12-atom spacer which minimizes steric hindrance effects during purification. The 6 percent beaded agarose and highly purified glutathione limit nonspecific binding and result in a high binding capacity of greater than 25mg of GST per milliliter of settled resin. The purification resin can be reused at least five times without loss in functionality.
The affinity purification of GST-tagged proteins using Pierce Glutathione Agarose is a simple, one-step process. Bound GST-tagged proteins are easily eluted with a buffer containing Thermo Scientific Reduced Glutathione. If the GST fusion protein contains a Factor Xa protease site, then the protein can be cleaved from the GST tag with Thermo Scientific Factor Xa.
Pierce Glutathione Agarose is available in numerous formats and as a standalone resin. Prepacked spin columns in a number of bed volumes are supplied alone or in kits. In addition, Pierce Glutathione Agarose comes in prepacked chromatography cartridges for FPLC applications.
For more information on Pierce Glutathione Agarose products, please call +1-815-968-0747 or visit www.thermoscientific.com/pierce.
Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.
About Thermo Fisher Scientific Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of more than $10 billion, we have approximately 35,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through two premier brands, Thermo Scientific and Fisher Scientific, which offer a unique combination of continuous technology development and the most convenient purchasing options. Our products and services help accelerate the pace of scientific discovery, and solve analytical challenges ranging from complex research to routine testing to field applications. Visit www.thermofisher.com.
