Frederick, MD: Syngene, a world-leading manufacturer of image analysis sol- utions, today announced the results of a head-to-head study carried out at the University of Cambridge in the UK to demonstrate that the G:BOX Chemi IR6 can produce images of IR labeled Western blots up to 10 times faster than laser based scanning.
The G:BOX Chemi IR6, which is Syngene’s new multi-application CCD imaging system for analyzing IR and fluorescently stained proteins, was tested against a laser based scanner. Both imagers were tested side-by-side in a comparison study at the University of Cambridge for analyzing proteins on Western blots labeled with DyLight® 680 and DyLight® 800 dyes (Thermo Fisher Scientific).
The results, detailed in Syngene’s Application Note 62, show that using the G:BOX Chemi IR6 with Epi Red lighting and a 705nm filter, an image of DyLight 680 labeled bands can be captured in just 20 seconds. With Epi LED IR740 lighting and a LY800 filter, an image of the DyLight 800 labeled proteins can be generated by the G:BOX Chemi IR6 in two minutes. In comparison, the laser based scanner can take more than 10 times longer to image DyLight 680 and twice as long to image DyLight 800. The systems had comparable sensitivity, with both their limits of detection being 0.11µg.
Lindsey Bunn, Syngene’s Application Specialist explained: “With laser-based scanners imaging times are determined by scanning resolution and intensity setting, whereas using a G:BOX Chemi IR6, imaging times only depend on sensitivity. This is why we believed our CCD system would be faster than and just as accurate as a laser based system and are delighted that the results of the study have proved this.”
Lindsey continued: “The added benefit with the G:BOX Chemi IR6 is the system can analyze all commercial fluorescent, chemiluminescent and visible dyes as well, making it a more cost-effective purchase. Researchers who need to rapidly produce images of multiplex labeled Westerns but whose labs don’t have the budget for an expensive laser scanner should consider testing a G:BOX Chemi IR6 system today.”
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About Syngene
Syngene is a world-leading supplier of integrated imaging solutions for analysis and documentation of gel-based information. Syngene’s systems are used by more than 10,000 research organizations and over 50,000 individual scientists world-wide and include many of the world’s top pharmaceutical companies and major research institutes.
Syngene, founded in 1997, is a division of the Synoptics Group based in Cambridge, UK. The Group’s other divisions, Syncroscopy and Synbiosis, specialize in digital imaging solutions for microscopy and microbial applications respectively. Synoptics currently employs 40 people in its UK and subsidiary operation in Frederick, USA.
