A Dolomite droplet generation system is helping researchers at Weill Cornell Medical College in New York, USA, to encapsulate human and mouse B cells for the cloning of antibody genes. Associate Professor Moonsoo Jin from the Department of Radiology explained: “Some of our immunology research is focused on cloning antibody genes. In this work, it is important to ensure that individual genes are isolated from each other, and that is why we have adopted the droplet technology. It enables us to encapsulate a single human or mouse B cell, guaranteeing that antibody genes from one cell are not mixed with those from another cell.”
“Biology itself is difficult enough, and so when we needed a microfluidic system for a new project we looked for a droplet generation system that was straightforward to use and not too complex. We chose Dolomite, investing in Mitos P-Pumps and a quartz Droplet Junction Chip. We’ve had the system for about two years now, and our experience has been very positive. It is simple to use and reliable, and we’ve been able to generate stable, uniform-size droplets able to withstand subsequent molecular biology procedures in a very reproducible and robust manner. We plan to continue our work with B cells and, in the future, may also use the droplet generation system to look at T cells and T cell receptor diversity and specificity.”
For more information, visit www.dolomite-microfluidics.com.
About Dolomite
Established in 2005 as the world’s first microfluidic application centre, Dolomite focused on working with customers to turn their concepts for microfluidic applications into reality.
Today, Dolomite is the world leader in solving microfluidic problems. With offices in the UK, US and Japan, and distributors throughout the rest of the world, its clients range from universities developing leading-edge analytical equipment, to manufacturers of chemical, life sciences and clinical diagnostics systems.
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