By contrast, placing a plate on BioCision’s thermo-conductive CoolSink and then putting the assembly on ice brings all wells to or below 4oC and the well-to-well temperature range was more uniform. The addition of an aqueous conductive medium (ACM) to replace air between the plate and CoolSink surface further enhanced results, with even faster equilibration to a final temperature of 3.3ºC, and less than 1ºC variation between wells.2
According to co-author Brian Schryver, BioCision Vice President, Research and Product Development, ‘CoolSink’s precision-engineered “direct contact” stage design eliminates the insulating air gap typically found between plate wells and the resting surface. The module’s excellent thermo-conductivity, due to its patented novel alloy construction, gives fast equilibration to below 4ºC, and using an ACM produces near identical well temperatures. This set-up provides a very simple way for researchers to ensure that all their samples are treated uniformly with no “plate edge effect”. CoolSink is an ideal tool for increasing the reproducibility of results in a variety of applications, such as cell-based assays, ELISA, FACS staining and analysis, migration or adhesion studies, temperature shift assays or any work with temperature-sensitive biomarkers.’
1 Brian Schryver and Rolf O. Ehrhardt, MD, PhD, ‘Improved thermal management of microtiter plates using the BioCision CoolSink’, BioTechniques 49:845–846, November 2010.
2 Brian Schryver and Rolf O. Ehrhardt, MD, PhD, ‘Improved thermal management of microtiter plates using the BioCision CoolSink’, BioTechniques 49:845–846, November 2010, Figure 2.
About BioCision
Headquartered in Mill Valley, California, USA, BioCision is the leading developer of portable benchtop tools for temperature-sensitive biomedical specimens and samples, for use in basic experiments, assays and clinical trials. Developed to provide temperature-sensitive sample management solutions, BioCision’s patented novel alloy sample modules enable standardised collection, organization, protection and high reproducibility of sample freezing, thawing and cooling. The advantages of ease of use, elimination of user error and very high reproducible performance have led to CoolProducts becoming the new standard for benchtop sample management.
BioCision CoolProducts have been adopted by leading pharmaceutical and biotechnology companies, many contract research organizations, and major academic and healthcare institutions worldwide.
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