After a little focusing up and down in a sample or cutting one thin section after another, every biologist wishes for one thing: deeper imaging. Ongoing advances in clearing agents, which make a sample more transparent, and microscope objectives that focus deeper into a sample give life scientists more information about biology’s structure. In addition, combining these tools with labeling adds molecular information to the picture, and innovations in light sources enable deeper penetration through tissue. In this article, experts from Carl Zeiss Microscopy (Oberkochen, Germany), Leica Microsystems (Mannheim, Germany), and Olympus America (Center Valley, PA) reveal some of the advances in deeper microscopic imaging.
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