Since its foundation, Creative Enzymes has extensive experience in enzyme manufacturing both for customers enzymes and scientific research and production of medicines, beer and other fields. In spite of this profound influence from its products, Creative Enzymes is still continuing to update its product line. This news is mainly about the hydrolasyses category.
Phospholipase A2
Phospholipase A2, also abridged into PLA2, is a common tool for hydrolyzing the ß-ester bond of zwitterionic glycerophospholipids. And its preferred substrates are phosphatidycholine, their plasmalogen analogues and phosphatidylethanolamine. And the phosphatidylinositol and phosphatidylserine are also hydrolyzed. In membranes of intact cells, Phospholipase A2 can attack phospholipids in membranes aggressively. The sn-a acyl bond of phospholipids specifically. What’s more, it also can act as a catalyst to hydrolyze the bond releasing arachidonic acid and lysophospholipids.
ß-Amylase
In polysaccharides of three or more a-(1,4) linked D-glucose units, ß-Amylase is used to hydrolyze the a-(1,4) glucan linkages. And its natural substrates, including starch and glycogen, will be broken down into glucose and maltose. Four isoenzymes with different isoelectric points are the main parts of pure, crystalline ß-Amylase preparation. In the absence of reducing agents, this enzyme can polymerize very rapidly through the sulfhydryl groups. p-Chloromercuribenzoate can inhibit the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can restore the activity completely.
Xylanase
Xylanase means a class of enzymes, which can degrade the linear polyasccharide beta-1,4-xylan into xylose, and then the hemicellulose can be broken down, which is one of the major components of plant cell walls. What’s more, it plays a very crucial role in micro-organisms in order to thrive on plant sources for the degradation of plant matter into usable nutrients. In many species, including fungi, bacteria, yeast, protozoans, snails and so on, this enzyme can be found.
“in order to meet customers’ all kinds of demands from academic and research use, Creative Enzymes always hold the commitment to updating new products with high quality.” said Dr. Derrick, a senior officer in Creative Enzymes.
With customers from all over the world and different research fields, Creative Enzymes’ newly-developed products will be welcome by a majority of scientists and its customers.