October, 2010 -- The manufacture and use of nanomaterials is increasing dramatically but there are serious concerns about the impact they may have on health and the environment.
One specific area of concern is how nanomaterials interact with cellular DNA perhaps causing damage and mutation. The Comet assay is one of several genotoxicity assays being used to measure nanoparticle induced DNA damage.
An increasing number of research groups conducting comet assays for this application use our Comet Assay scoring systems (http://www.perceptive.co.uk/cometassay/). The following peer reviewed articles have recently been published.
Papers
Gene toxicity studies on titanium dioxide and zinc oxide nanomaterials used for UV-protection in cosmetic formulations (2010) R. Landsiedel et al, Nanotox. 4(4):364-381
In vitro evaluation of SiC nanoparticles impact on A549 pulmonary cells: cyto-, genotoxicity and oxidative stress (2010) S. Barillet et al, Toxicol. Letters 198(3):324-330
Thresholds for indirect DNA damage across cellular barriers for orthopaedic biomaterials (2010) M Parry et al, Biomaterials 31(16):4477-4483
Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells (2009) K. Gerloff et al, Nanotoxicology 3(4):355-364
Delphinidin Modulates the DNA-Damaging Properties of Topoisomerase II Poisons (2009) M. Esselen et al, Chem. Res. Toxicol. 22(3):554-564
Genotoxicity evaluation of locally produced dental porcelain – An in vitro study using the Ames and Comet assays (2009) M. Noushad et al., Toxicol In Vitro. 23(6):1145-50
Nanoparticles can cause DNA damage across a cellular barrier (2009) G. Bhabra et al., Nature Nanotech. 4(1): 876-883
SiC nanoparticles cyto-and genotoxicity to Hep-G2 cells (2009) S.Barillet et al J. Phys.: Conf Ser. 170 012016
To view these papers please visit our Recent Papers page (http://www.scorecomets.com/assay/#papers).
About Perceptive Instruments
Perceptive Instruments Ltd. (http://www.perceptive.co.uk) develops, manufactures and supplies image analysis and data processing solutions for many areas of scientific research and industry. Our products are used primarily in the pharmaceutical & health care industries, as well as environmental monitoring, food and manufacturing industries. We have established an enviable reputation based upon our willingness and ability to completely satisfy our clients’ needs. We provide standard ‘off the shelf’ systems and customised solutions based on current or novel technologies. Examples of special solutions include systems for antibiotic testing, vaccine development and robot vision.
Our Products Comet Assay IV Comet Assay IV (http://www.perceptive.co.uk/cometassay/) is an interactive live video-based system for scoring cells subjected to the single cell gel electrophoresis technique (comet assay). The system is the very latest in a highly successful series of comet assay scoring systems from Perceptive Instruments that have evolved and developed since the release of our first system in 1992.
Sorcerer Colony Counter Sorcerer Colony Counter (http://www.perceptive.co.uk/colony-counter/) provides instant, accurate plate counting for microbial and mammalian colonies. It is ideal for laboratories engaged in drug development and production, food and milk testing, environmental monitoring and public health.
Ames Study Manager The Ames Study Manager (http://www.perceptive.co.uk/amesstudymanager/)is a study management and reporting system for Ames testing. Together with the Sorcerer automatic colony counter it provides a complete solution for regulatory and non-regulatory safety assessment.
SpeckCheck 2 SpeckCheck 2 (http://www.perceptive.co.uk/speckcheck2/) is the advanced scanner based system for automatic detection and analysis of dirt and other contaminants in pulp and paper.
Contact Us Web: http://www.perceptive.co.uk Email: sales@perceptive.co.uk Tel: +44 1440 730773
Perceptive Instruments Ltd Blois Meadow Business Centre Steeple Bumpstead Haverhill CB9 7BN UK Tel. +44 (0)1440 730773 Fax. +44 (0)1440 730630 www.perceptive.co.uk
