Rapid hot-start activation and processive enzymes make GoTaq Probe Systems compatible with any real-time PCR instrument using standard or fast cycling programs. The 1-step and 2-step systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. These Systems are compatible with other probe assays such as molecular beacons.
GoTaq Probe qPCR Master Mix, designed for qPCR from gDNA templates, provides resistance to a wide range of PCR inhibitors. Using antibody-mediated hot-start chemistry, reaction set-up can be performed at room temperature. The master mix does not contain a reference dye, however carboxy-X-rhodamine is included with the system, to be added to amplification reactions if desired.
GoTaq Probe 1-Step RT-qPCR System combines GoScript Reverse Transcriptase and GoTaq Probe qPCR Master Mix in single-step, real-time amplification reactions for the detection and relative quantification of RNA expression levels using a one-step RT-qPCR method.
GoTaq Probe 2-Step RT-qPCR System enables detection and relative quantification of RNA expression levels via a two-step RT-qPCR method, using the integrated components of the GoScript Reverse Transcription System and GoTaq Probe qPCR Master Mix. GoScript Reverse Transcription System includes an optimized reaction buffer and reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product may be added directly to downstream qPCR amplification reactions.
For more information about the GoTaq Probe Real-Time PCR Systems, please visit: www.promega.com/gotaqprobe
For a high resolution image please contact Zyme Communications katie.odgaard@zymecommunications.com
Enquiries:
Katie Odgaard
Zyme Communications Ltd
Tel: +44 (0) 7787 502 947
Skype: kodgaard
Email: katie.odgaard@zymecommunications.com
www.zymecommunications.com
Penny Patterson
Sr. Director Communications
Promega Corporation
Phone: +1 (608) 274-4330
E-mail: penny.patterson@promega.com
www.promega.com
About Promega
Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. The company's 2,000 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, molecular diagnostics and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA, with branches in 15 countries and over 50 global distributors. For more information about Promega, visit: www.promega.com
