The first poster, “A new non-fluorescent quencher for use in oligonucleotide synthesis”, describes the creation of a new quencher to ease the preparation of probes for use in assays where the fluorophore emits in the 550 to 600nm range. Entitled “Cyanine functionalised solid supports for oligonucleotide synthesis”, the second poster discusses the benefits of incorporating cyanine dyes at the 3’ end of an oligonucleotide via the use of solid CPG supports. The greatest advantage of the method is that it eliminates the need for post-synthetic labelling onto an oligonucleotide modified with an amino linker at the 3’ end. Dual cyanine dye labelled probes (i.e. cyanine 540/cyanine 650) are especially useful for detecting DNA-protein interactions using FRET.
Link Technologies’ extensive range of products includes a wide choice of phosphoramidites and CPG solid supports for synthesising oligonucleotides. These include those labelled with fluorescent dyes such as TAMRA (rhodamine), fluorescein (including 6-FAM, HEX and TET) and Quasar™ Dyes, as well as the dark quenchers dabcyl, Deep Dark Quencher 1 and the Black Hole Quenchers™.
For more information please visit the Link Technologies website at www.linktech.co.uk or follow us on Twitter: @linktechdna.
About Link Technologies Ltd
Link Technologies Ltd is a leading kilo-scale supplier of speciality reagents for oligonucleotide synthesis and modification, all to the ISO 9001:2008 quality certification. The company’s main markets are university and commercial research departments, plus, increasingly, larger Contract Manufacturing Organisations (CMOs) in the biotech sector. Link has also forged successful links to academia working closely with many UK universities. In tandem with internal research and development, the Company has an excellent record in bringing in-licence products to market with various worldwide commercial partners.
For further information see: www.linktech.co.uk.
