LINCOLN, Neb., Aug. 20 /PRNewswire/ -- Researchers at Stanford University are using the advantages of infrared imaging to benefit their research on pathogens interacting with epithelial cells. Drs. Manuel Amieva and Roger Vogelmann are studying the effect of H. pylori on tight barrier function and cell polarity in epithelial cells and are using the Odyssey(R) Infrared Imaging System from LI-COR Biosciences to aid their research.
They have adapted the Odyssey to benefit their research by developing several unique infrared assays including measuring the barrier function of tight junctions in mammalian epithelial cells to identify bacterial clones that are positive for certain proteins, and to determine the amount of bacteria attached to epithelial cells.
Using the infrared detection system of the Odyssey, Amieva and Vogelmann were able to do their studies more efficiently and with less exposure to radioactive material. “We are now able to identify positive bacterial clones very specific on the bacterial plate and we don’t have to screen a multitude of clones,” says Vogelmann. “Also the Odyssey system abolishes endless hours of counting bacteria attached to epithelial cells. With the new system we can determine the amount of attached bacteria rapidly using the immunofluorescence based assay. The quantification of fluorochrome labeled tracers and antibodies (e.g. in regular IB) allowed us to move away from radioactive material almost completely.”
A paper detailing their research, and procedures is now available at http://www.licor.com/ecr.
The Odyssey Infrared Imaging System is uniquely equipped with two infrared channels for direct fluorescence detection on membranes with sensitivity that is equal to or better than chemiluminescence. The superior signal-to-noise ratio produced by the near infrared dyes produces results not possible with traditional fluorescence-based imaging systems.
LI-COR Biosciences is a leader in the design, manufacture, and marketing of integrated instrument systems for plant biology, biotechnology, drug discovery, and environmental research. The company pioneered the development of infrared fluorescence labeling and detection systems for DNA sequencing, protein imaging, genotyping, and AFLP(R) for genomic and proteomic research. LI-COR instruments for photosynthesis, carbon dioxide analysis, and light measurement are recognized world wide for standard-setting innovation in plant science research and environmental monitoring.
Founded in 1971, the privately held company is based in Lincoln, Nebraska, with subsidiaries in Germany and the United Kingdom. LI-COR systems are used in over 100 countries and are supported by a global network of offices and distributors.
AFLP is a registered trademark of Keygene, N.V.
LI-COR Biosciences
CONTACT: D. Doc Chaves, Marketing Communications Manager of LI-CORBiosciences, +1-402-467-0750, dchaves@licor.com
Web site: http://www.licor.com/ecr
