San Jose, CA – May 13, 2009 AnaSpec, the provider of the world’s largest collection of ready-to-go ?-amyloid peptides and related products for Alzheimer’s disease (AD) research, has introduced a line of ?-amyloid peptides labeled with biotin (N or C-terminal biotin labels) as well as N-terminal biotin labeled ?-amyloid peptides with a fluorescein label on the C-terminus.
These peptides have been used in different applications. Nelson, TJ and Alkon, DL. used biotin-LC-?-amyloid-(1–42) in A?-proteins binding studies.1 A?-proteins complex were precipitated with avidin-agarose and western blots run. A paper by Bohrmann, B. et al. reported the use of biotin-A?(1-40)0) or biotin-A? (1-42) for their A? polymerization studies. 2 Biotin-A??(1-40) or biotin-A? (1-42) peptides were incubated in A??(1-40)?or A? (1-42) coated plates, and streptavidin-peroxidase added. Color development was done using tetramethyl-benzidin, a chromogenic substrate for peroxidase. In the same paper,2 biotin-A? ( (1-40) incorporation into preformed A??(1-42)?fibrils was reported. Biotin-A??(1-40) was added to a low concentration of A? (1-42) fibrils and stained with anti-biotin Ig labeled with colloidal gold and negatively stained with uranyl acetate. A paper by Liu, Y. et al shows the use of biotinylated fibrillar A??(1-42) in electron microscopy.3
Using what they called “FA?B,” fluorescein beta-amyloid (1-40)-Lys(LC-biotin), Leissring, MA. et al. performed proteolysis measurement using fluorescence polarization (FP) technique.4
References:
1. Nelson, TJ. and DL. Alkon, J. Biol. Chem. 282, 31238 (2007).
2. Bohrmann, B. et al. J. Biol. Chem. 274, 15990 (1999).
3. Liu, Y. et al. Brain 128, 1778 (2005).
4. Leissring, MA. et al. J. Biol. Chem. 278, 37314 (2003).
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