The Pierce® Direct IP Kit procedure requires only 2-10 micrograms of purified antibody, but is easily scalable. The short coupling method (1-2 hours) covalently attaches antibody to the resin. This irreversible attachment results in minimal contamination of the purified protein and allows the resin to be reused several times. The purified protein obtained using the kit is compatible with mass spectrometry and other downstream applications such as enzymatic assays.
The Pierce Direct IP Kit includes convenient spin columns that eliminate resin loss and provide for efficient separation of solutions. The kit also supplies cell lysis buffer and sufficient reagents to perform at least 50 antibody immobilizations and IP experiments using 10 microliters of immobilized antibody support.
The Pierce Direct IP Kit, along with the Pierce Classic IP and Pierce Crosslink IP Kits, is a new family of products featuring improved immunoprecipitation methods. For more information on these kits, visit www.thermo.com/pierce or call +1-815-968-0747.
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About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10.5 billion, we have approximately 34,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. The Thermo Scientific brand represents a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com.
