This rapid protocol is considerably faster than traditional ELISA kits but maintains a high signal-to-noise ratio. The assay accurately characterizes antibodies from hybridoma cell culture supernatants and ascites fluid, as well as purified antibodies.
The Pierce® Rapid ELISA Mouse mAb Isotyping Kit uses an 8-well strip format, with individual wells pre-coated with anti-mouse heavy-chain capture antibody (anti-IgG1, IgG2a, IgG2b, IgG3, IgA and IgM) or anti-mouse light-chain capture antibody (kappa or lambda). This eliminates the need to purify and immobilize an antigen for isotyping. Results can be assessed visually or measured quantitatively at 450 nm using a standard ELISA plate reader.
The cost-effective kit includes pre-coated plates, detecting antibody, buffers, and TMB substrate and stop solutions, with sufficient reagents to isotype 60 mouse monoclonal antibodies. For more information on the Thermo Scientific Pierce Rapid ELISA Mouse mAb Isotyping Kit, visit www.thermo.com/pierce or call +1-815-968-0747.
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About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10.5 billion, we have approximately 34,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. The Thermo Scientific brand represents a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com.
