Integrated DNA Technologies Increases qPRC Sensitivity and Specificity with its Double-Quenched(TM) Probes

April 28, 2011 |

1 min read

CORALVILLE, IA (28th April 2011) – Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, offers its double-quenched probe, to increase the accuracy and reliability of 5’ nuclease qPCR experiments. This novel proprietary probe design positions an internal ZEN™ quencher 9 bases from the 5’ fluorophore, which when combined with the standard 3’ quencher, significantly decreases background fluorescence and increases sensitivity. Due to the incorporation of ZEN, the initial background fluorescence signal is much lower, making subsequent changes easily detectable while assay sensitivity is increased. As a result low background is obtained, even at 40 base pairs or longer, thus increasing specificity without any loss in sensitivity or quenching efficiency.

This improved functionality significantly increases qPCR accuracy and sensitivity when compared to traditional probes. Download the ZEN double quenched probe datasheet here.

For further information, or to tell us about your ZEN moments, please visit http://www.idtdna.com/primetime.

About IDT

Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; and Leuven, Belgium. For more information visit www.idtdna.com.

Integrated DNA Technologies

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