Creative Biolabs has established a platform for industrial scale production of recombinant IgG in mammalian cells. In order to mimic the behavior of a recombinant human IgG antibody targeting a human protein in humans better, specificity-matching recombinant mouse IgG antibodies derived from phage display mouse scFv/Fab libraries targeting the mouse protein are frequently produced and used in animal models by Creative Biolabs. They use a modified FRT/Flp-In recombinase system to produce human IgG in CHO cells for stable expression of humanized/recombinant human IgG. The same approach is also used in stable expression of recombinant mouse IgG. They have pre-selected CHO cell lines that contain a single stably integrated FRT site at a transcriptionally active genomic locus and they have proven that these cell lines can produce higher level expressers of recombinant human IgGs targeting various antigens. Of course, even starting from these pre-selected cell lines, significant amount of time and effort are still required to create a stable cell line that can express recombinant human/mouse IgG at a cost-effective level, say 800mg/L. In the end of production, the recombinant antibody will be purified in three steps (protein A/G followed by two polishing steps, antibody monomer >99%, endotoxin level < 0.5 EU/mg antibody, protein A residual < 10 PPM, which is equivalent to antibody for human use). The cell lines will be archived for two years free of charge to client, in case future production is needed.
Construction of chimeric antibody expression vectors
The gene of a scFv or Fab antibody obtained from phage display technology or amplified from a mouse/rat hybridoma cell line is cloned into their mammalian expression vectors that contain the constant regions of human or mouse IgG light chain and heavy chain.
Transient transfection of CHO cells
Transient expression using CHO or 293 cells will be performed to confirm the expression of the recombinant antibody. For small scale antibody production, transient expression is more inexpensive in comparison with stable expression.
Creating stable CHO cell lines
Stably transfected CHO cell line are screened to select high expressers. The production cell lines are adapted into protein-free culture medium for bio-manufacturing. The gene-engineered CHO cells they have allow the establishment of CHO expressers with extremely high yield, which will dramatically reduce the cost in production of full-size recombinant IgG. Creative Biolabs once achieved a cell line with a yield of 4.5g/L. Learn more about recombinant IgG production at http://www.creative-biolabs.com/recombinant-igG-production.html.
About Creative Biolabs
Creative Biolabs is a recognized service provider in converting small gene-engineered scFv or Fab human/mouse antibodies derived from phage display antibody library screening or mouse/rat hybridoma cell lines into full-size recombinant human or mouse IgGs with Fc fragments of various functions, e.g. ADCC, or non-ADCC. Email: contact@creative-biolabs.com Tel: 1-631-871-5806 Fax: 1-631-207-8356
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Construction of chimeric antibody expression vectors
The gene of a scFv or Fab antibody obtained from phage display technology or amplified from a mouse/rat hybridoma cell line is cloned into their mammalian expression vectors that contain the constant regions of human or mouse IgG light chain and heavy chain.
Transient transfection of CHO cells
Transient expression using CHO or 293 cells will be performed to confirm the expression of the recombinant antibody. For small scale antibody production, transient expression is more inexpensive in comparison with stable expression.
Creating stable CHO cell lines
Stably transfected CHO cell line are screened to select high expressers. The production cell lines are adapted into protein-free culture medium for bio-manufacturing. The gene-engineered CHO cells they have allow the establishment of CHO expressers with extremely high yield, which will dramatically reduce the cost in production of full-size recombinant IgG. Creative Biolabs once achieved a cell line with a yield of 4.5g/L. Learn more about recombinant IgG production at http://www.creative-biolabs.com/recombinant-igG-production.html.
About Creative Biolabs
Creative Biolabs is a recognized service provider in converting small gene-engineered scFv or Fab human/mouse antibodies derived from phage display antibody library screening or mouse/rat hybridoma cell lines into full-size recombinant human or mouse IgGs with Fc fragments of various functions, e.g. ADCC, or non-ADCC. Email: contact@creative-biolabs.com Tel: 1-631-871-5806 Fax: 1-631-207-8356
Help employers find you! Check out all the jobs and post your resume.
