Carl Zeiss’ Dual Camera Module Captures Multichannel Images Simultaneously with Maximum Resolution

Thornwood, NY - The Dual Camera module for the AxioVision image analysis software from Carl Zeiss is intended for all users in the life sciences, including cell biologists, virologists and physiologists who examine fast intracellular processes with maximum resolution in both time and space. The module provides the capability to simultaneously acquire images with two cameras and their highly precise synchronization within nanoseconds. The camera parameters such as exposure time or contrast can be set independently.

Dual Camera applications are comprised of two identical cameras with adapter, suitable filters and a software module. Capturing images of biological samples with two different wavelengths in two channels permits the measurement of emission ratio imaging (Indo-1), fast FRET (Förster Resonance Energy Transfer) examinations and the imaging of cellular transport processes in cell cultures, tissues or organisms. Simultaneously imaging tissue and cell structures using infrared transmitted-light techniques like IR-DIC (differential interference contrast) and fluorescence excitation – a major requirement for electrophysiological work in neurobiology – is a further application that benefits from the Dual Camera module.

Due to the simultaneous capture of two separate images, the Dual Camera module provides a higher speed of capture than techniques where images are only captured sequentially with one camera. Furthermore, artifacts that can occur in sequential captures of double-stained structures with only one camera are effectively prevented, as are errors in ratiometric measurements of two emission channels (e.g. with Indo-1).

Thanks to Dual Camera, it is no longer necessary to mechanically change filters or to use emission filter wheels to visualize two dyes. Additionally, projecting two images on the sensor of a camera via optical adapters using Dual Camera does not sacrifice the field of view. The functionality of the AxioVision Physiology recording and analysis module, in particular, benefits from the possibilities provided by Dual Camera. The Dual Camera module is also an attractive supplement to the Cell Observer SD confocal recording system.

Together with the Cell Observer HS and Cell Observer SD microscope systems, Dual Camera creates an ideal system for live cell imaging and physiology.

Photo: The Dual Camera module for the AxioVision image analysis software from Carl Zeiss permits the examination of fast intracellular processes with maximum resolution in both time and space.

Text and photos on the Internet: www.zeiss.de/micro-press.

About Carl Zeiss

Carl Zeiss MicroImaging, Inc., a subsidiary of Carl Zeiss, Inc., offers microscopy solutions and systems for research, laboratories, routine and industrial applications. In addition, Carl Zeiss MicroImaging markets microscopy and digital pathology systems for the clinical market, as well as spectral sensors for industrial and pharmaceutical applications. Since1846, Carl Zeiss has remained committed to enabling science and technology to go beyond what man can see. Today, Carl Zeiss is a global leader in the optical and opto-electronic industries.

With 11,249 current employees in the Group and offices in over 30 countries, Carl Zeiss is represented in more than 100 countries with production centers in Europe, North America, Central America and Asia. For more information on the breadth of solutions offered by Carl Zeiss MicroImaging, please visit www.zeiss.com/micro.

MORE ON THIS TOPIC