Research Article Cites Cleanascite™ for microRNA Isolation
MONMOUTH JUNCTION, NJ, June 1, 2016 -- Biotech Support Group reports on a recent research article describing the simplicity and efficiency of their proteomic sample preparation technology for clearing lipids from egg yolk.
In brief, the article’s authors report a method for the reproducible and reliable isolation of miRNA from the albumen and yolk of chicken eggs. These methods will allow the investigation of epigenetic programming in chick development previously unknown, and how this impacts the nutritional value of eggs for human consumption. The article states "…400 µl aliquots of the yolk/lysis solution was dispensed into five 1.5 ml microcentrifuge tubes. To each of these aliquots 600 µl of Cleanascite™ was added followed by rigorous vortexing until the sample became homogenous. The Cleanascite™ removes the lipid from this high fat tissue that would otherwise interfere with the extraction process…Solutions were then incubated at 4 °C for 1 h.”.
The citation is:
Ben Wade, Michelle Cummins, Anthony Keyburn and Tamsyn M. Crowley. Isolation and detection of microRNA from the egg of chickens. BMC Research Notes 2016 9:283. DOI: 10.1186/s13104-016-2084-5 (https://bmcresnotes.biomedcentral.com/articles/10.1186/s1...)
“This is still another example of the versatility of Cleanascite™ as it now shown to help purify microRNA from egg yolk, a very lipid-rich tissue type. The exquisite selectivity profile of Cleanascite™ makes this possible.” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at http://www.biotechsupportgroup.com/Cleanascite-Lipid-Remo...
About Biotech Support Group LLC
Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NuGel™ for functional proteomics. From these innovations, the company has acquired knowledgebase and biomarker intellectual property assets that support discoveries of protein markers from blood, with special emphasis on early detection and personalized medical decisions for cancer patients. For more information, go to http://www.biotechsupportgroup.com
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