ST. LOUIS, March 3 /PRNewswire-FirstCall/ -- Sigma, a division of Sigma-Aldrich Corporation , has launched a new HIS-Select(TM) iLAP(TM) High Capacity 96-Well plate (Product code H 9412). The innovative, new iLAP (Integrated Lysis and Affinity Purification) plate is a patent-pending product that combines the cell lysis and HIS-tagged protein purification steps into one. This exclusive new plate reduces the number of steps and offers substantial timesaving for high throughput purification of HIS-tagged proteins. In addition, the HIS-Select technology of the plate provides high specificity and binding capacity for histidine containing fusion proteins.
Sigma’s HIS-Select(TM) iLAP(TM) HC 96-well Plates are coated with both a cell lysis reagent and the HIS-Select nickel chelate matrix. This technology allows for cells to be lysed and the HIS-tagged protein to be captured all in one step. Cell lysis and protein extraction is highly efficient and eliminates the need to harvest cells from the culture. The HIS-Select coating is highly selective for HIS-tagged proteins, reduces non-specific binding, and has a high binding capacity.
“The HIS-Select iLAP plate has dramatically simplified high-throughput purification of HIS-tagged proteins,” commented Michael Hadjisavis, Manager of Strategic Business, Protein Expression and Proteomics, at Sigma-Aldrich. “Because this plate allows for simultaneous cell lysis and protein capture, it will significantly reduce the screening time of 96 recombinant clones for protein production from an entire day to two hours. It will also simplify other high throughput applications for characterizing HIS-tagged proteins like protein:protein interaction assays.”
Sigma-Aldrich is a leading Life Science and High Technology company. Our biochemical and organic chemical products and kits are used in scientific and genomic research, biotechnology, pharmaceutical development, the diagnosis of disease and chemical manufacturing. We have customers in life science companies, university and government institutions, hospitals and in industry. Over one million scientists and technologists use our products. Sigma-Aldrich operates in 34 countries and has 5,900 employees providing excellent service worldwide. We are committed to the success of our Customers, Employees and Shareholders through leadership in Life Science, High Technology and Service.
This release contains forward-looking statements relating to future performance, goals, strategic actions and initiatives and similar intentions and beliefs and other statements regarding the Company’s expectations, goals, beliefs, intentions and the like, which involve assumptions regarding Company operations and conditions in the markets the Company serves. The Company does not undertake any obligation to update these forward-looking statements.
To request information about HIS-Select(TM) iLAP(TM) Plates and other HIS- Select products, please visit sigma-aldrich.com/hisselect .
Sigma-Aldrich Corporation
CONTACT: Michael Hadjisavis, +1-314-286-7431, or Kim Harper,+1-314-286-8374, both of Sigma-Aldrich Corporation
