Fremont CA – Oct 2, 2012 -- AnaSpec is pleased to introduce the newest member of the Caspase Kits series – the SensoLyte® 520 Generic Caspase Assay Kit, and showcase our other apoptosis product offering. This includes a suite of SensoLyte® Caspase and Cytotoxicity Assay Kits, caspase and apoptosis related peptides, and antibodies, including zebrafish specific antibodies.
This kit is ideal for in situ detection of caspase activity in cells undergoing apoptosis using a fluorochrome inhibitor of caspase, FAM-VAD-FMK. This reagent is cell permeable and non-cytotoxic. When added to a population of cells, it enters cells and covalently binds to activated caspases-1, -3 -4, -5, -7, -8, and –9. Bound FAM-VAD-FMK is retained within the cell, while any unbound FAM-VAD-FMK diffuses out of the cells and is washed away. Signal in the cells containing the bound FAM-VAD-FMK can be evaluated with a fluorescence plate reader, fluorescence microscopy, or flow cytometry. The SensoLyte® Caspase Assay Kits are one-step homogeneous assays. Designed in a convenient mix-and-read format, and containing highly sensitive fluorimetric components, SensoLyte® Caspase Assay Kits are an ideal solution for high-throughput screening.
References:
1. Thornberry, NA. and Y. Lazebnik, Science 281, 1312-1316 (1998).
2. Reed, JC. J. Clin. Oncol. 17, 2941-2953 (1999).
3. Lazebnik, YA. et al. Nature 371, 346-347 (1994).
4. Villa, P. et al. Trends Biochem. Sci. 22, 388-393 (1997).
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AnaSpec, EGT Group
AnaSpec, EGT Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and qPCRs. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.
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