Amongst dye reagents, including BCECF, AM and Carboxy-fluorescein diacetate, Calcein AM is the most suitable indicator for staining viable cells due to its low cytotoxicity property. Calcein AM does not affect cell proliferation or viability.2-3 Calcein AM is used in cell invasion, adhesion, migration and other cell-based assays.
AnaSpec is pleased to provide Calcein AM at two purity grades, >90% or >95%, as determined by HPLC. AnaSpec also offers the SensoLyte® Calcein Cell Viability Assay Kit - a rapid and sensitive assay to detect viable cells. This kit provides ample reagents (dye and assay buffer) for 1000 assays in a 96-well plate format. For bulk quantity purchase, please email service@anaspec.com.
AnaSpec’s Calcein AM, a green fluorescent cell viability indicator, has excellent quality, and is cost-effective.
References
1. Braut-Boucher, F. et al. J. Immunol. Methods 178, 41 (1995).
2. Akeson, AL. et al. J. Immunol. Methods 163, 181 (1993).
3. Weston , SA. et al. J. Immunol. Methods 133, 87 (1990).
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AnaSpec, Eurogentec Group
AnaSpec, Eurogentec Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and qPCRs. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.
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