Aliso Viejo, CA – March 20, 2009: Ambry Genetics today announced capabilities of performing sequence capture for the Illumina® or Roche® Next Gen Sequencing platforms. Customers will have the option of choosing either custom 385K or pre-designed 2.1M high density arrays available through Roche NimbleGen. Sequencing can be done in-house at Ambry on the Illumina® GA II, or returned to the customer for sequencing on their platform.
“We are pleased to be offering these services to our customers”, Anja Kammesheidt, Ambry’s CSO states.”Sequence capture in conjunction with Next Gen Sequencing provides a powerful tool kit that allows us to look at megabases of sequence in a few steps without having to go through cumbersome gene specific or long range PCR. The microarrays are used similar to a magnet to capture regions of interest. Subsequently, via sequencing-by-synthesis one is able to generate simultaneous sequence reads from millions of surface amplified DNA fragments.”
In this process, a sample is prepared for sequencing by shearing of genomic DNA, hybridization of the fragments against custom or predesigned oligo tiling arrays containing candidate regions, followed by washing steps which remove non- specific targets and a final elution of the target DNA. The eluted amplified fragments are called a library, which is used to create clusters, surface-bound colonies of DNA, for example on the surface of the Illumina® GAII flow cell. These clusters are then sequenced on the genome analyzer using polymerase and differently labeled fluorescent nucleotides.
Currently, sequences generated are typically 36-54 bp long, for a total of up to ~1.0 GB per lane of each flow cell with paired-end sequencing, even though longer read lengths can be achieved. Data is aligned back to reference sequences and scored for coverage, SNPs, and mutations.
About Ambry Genetics
Ambry Genetics is a CAP-accredited, CLIA-certified commercial clinical laboratory headquartered in Aliso Viejo, California. Since the company’s inception in 2000, it has become a leader in providing genetic services focused on clinical diagnostics, pharmacogenomics and research support. Ambry has built a solid reputation for unparalleled service, and has been at the forefront of applying new technologies. To learn more about testing and services available through Ambry, visit www.ambrygen.com.
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