EPSOM, UK (December 03, 2009) Thermo Fisher Scientific Inc., the world leader in serving science, today announced the new Thermo Scientific Verbatim High Fidelity DNA Polymerase. This unique, advanced DNA polymerase allows highly accurate amplification of DNA templates, equivalent to industry leading proofreading enzymes, while maintaining processing speed and delivering high yields. These high-performance characteristics make Verbatim High Fidelity DNA Polymerase ideal for demanding downstream applications such as cloning, site-directed mutagenesis and sequencing.
High Fidelity PCR requires a DNA polymerase with proofreading capabilities, and should be used in applications requiring accurate amplification of a DNA target. Thermo Scientific demonstrated the advanced proofreading capabilities of Verbatim High Fidelity DNA Polymerase during in an in-house comparative evaluation. Verbatim demonstrated its industry leading performance by delivering greater enzyme fidelity than all other enzymes tested1 (Taq DNA polymerase, Pfu DNA polymerase*/Taq blend, KOD DNA polymerase* and a leading fusion enzyme).
Verbatim High Fidelity DNA Polymerase also demonstrates increased affinity for DNA templates, which significantly improves yield and processivity. This increased processivity allows PCR protocol times to be reduced by up to 75 percent, for faster results. Enhanced DNA binding affinity also ensures that Verbatim is suitable for a broad range of templates, and the enzyme is supplied with two optimised buffering systems - one for standard templates and the other for more complex targets (such as GC-rich templates).
Thermo Scientific Verbatim High Fidelity DNA Polymerase kits (with sufficient reagents for 200 or 1000 reactions) are available with or without dNTPs. For further information, speak to your Thermo Fisher Scientific representative or visit www.thermo.com/gen.
Pfu DNA polymerase (derived from Pyrococcus furiosus) and KOD DNA polymerase (derived from Thermococcus kodakaraensis) are commonly used proofreading enzymes. These enzymes contain a 3’ – 5’ exonuclease activity that is referred to as ‘proofreading activity’. Their processivity is much lower than Taq DNA polymerase, which leads to longer cycle times and lower yields.
References: 1. Data on file, Thermo Fisher Scientific
Thermo Scientific is part of Thermo Fisher Scientific Inc., the world leader in serving science.
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With 2008 revenues of $10.5 billion, we have approximately 34,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com.
