Using a specialised and improved algorithm developed exclusively for Olympus, the deconvolution solution module provides advanced, high-speed image restoration. Maximising modern PC computing capabilities, it leverages multi-core processor architectures to provide an extremely powerful deconvolution facility, enabling the sharpening of even the most blurred images. Consequently, users can obtain a fast and easy-to-use, comprehensive deconvolution, removing the complex parameter adjustment steps required with other advanced de-blurring algorithms.
The resulting high-quality image stacks can be visualised in three dimensions, using the powerful VoxelViewer to display structures and ISO projections via a simple click of the mouse. This enables more in-depth analysis, with the capability to display stereo adaptations of the image. Furthermore, the online de-blur functionality enables users to visualise the deconvoluted images during the live operation of the camera.
A broad range of image types can be deconvolved using the Olympus deconvolution solution module, including fluorescence, confocal and brightfield. With the additional capability to perform blind deconvolution, the software is able to use a theoretical point spread function (PSF), adapting it to the specific data. Furthermore, the PSF calculation can be saved and applied when performing subsequent deconvolutions. In addition, images obtained from non-Olympus systems are also easily imported, making it a highly versatile imaging solution.
cellSens Dimensions
Designed to offer control and processing for microscope-based experimental systems, the cellSens Dimensions software package provides a broad range of advanced features, as well as specialised, optional solution modules. Users can conduct a range of complex and highly sophisticated experiments, from extended focal imaging to multiple image alignment and multi-position imaging. In addition, live images can be transferred directly to the web using the netcam functionality, enabling rapid discussion with colleagues, wherever they are in the world. With the ability to control a wide range of Olympus and non-Olympus hardware, advanced and precise time-lapse experiments can be conducted, for example. The resulting time stacks, can even be put through kinetic and threshold-based object analysis. Once data has been obtained, the unique report composer uses Microsoft Word templates to generate user-defined reports that retrieve images and data directly from the cellSens database.
Image caption: Comprehensive constraint iterative deconvolution for crisp and detailed results. C. Elegans hermaphrodite in the late L2 stage. Green: PDZ-protein TAG-60. Expression detected on the lumenal side of intestinal cells. Red: protein AJM-1, which is expressed in the junctions of epithelial cells. Blue DNA stained by DAPI.
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