PENZBERG, Germany--(BUSINESS WIRE)--In a recent study initiated by Beiersdorf AG (1), Florian Spörl and colleagues evaluated the use of Roche Applied Science’s (Pink Sheets: RHHBY) (SWX:RO) (SWX:ROG) xCELLigence System as a viable system to monitor not only cholesterol extraction and repletion but also cholesterol reorganization in human keratinocytes in real time. The xCELLigence System offers a novel approach to non-invasive long-term observation of membrane cholesterol dynamics in primary human keratinocytes. This real-time cell analysis system measures alterations in cellular impedance using microelectrodes on the surface of a 96-well culture plate (E-Plate). Spörl et al. were able to analyze the effects of membrane cholesterol reorganization on proliferation and differentiation, and their findings imply a strictly controlled mechanism for the regulation of membrane cholesterol composition in both early and late keratinocyte differentiation.
