In the workshop, Dr Rose will present data demonstrating how PrimeTime Predesigned qPCR Assays with ZEN double-quenched probes simplify a 4-plex assay system, saving time and money, and discuss factors that influence the success of using these assays in a multiplex format. The 15 minute technology spotlight will introduce IDT’s new multi-application molecular biology tool, gBlocks™ Gene Fragments. These double-stranded, sequence-verified DNA fragments up to 500 base pairs in length can be used as copy number standards for qPCR, without the need for cloning the endogenous amplicon. Multiple amplicons can be incorporated into a single gBlocks fragment, avoiding the need for linearizing plasmid controls and ensuring that concentrations of the standard dilutions are the same for the individual control assays in the multiplex reaction.
To register to attend Dr Rose’s workshop and learn more about the Technology Spotlight, visit the Genomics Research Europe website for further details. For more information on IDT’s qPCR technologies, please visit www.idtdna.com. Follow us on twitter @idtdna for real-time updates and insights.
About IDT
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; and Leuven, Belgium. For more information visit www.idtdna.com.
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