This kit is ideal for in situ detection of caspase activity in cells undergoing apoptosis using a fluorochrome inhibitor of caspase, FAM-VAD-FMK. This reagent is cell permeable and non-cytotoxic. When added to a population of cells, it enters cells and covalently binds to activated caspases-1, -3 -4, -5, -7, -8, and –9. Bound FAM-VAD-FMK is retained within the cell, while any unbound FAM-VAD-FMK diffuses out of the cells and is washed away. Signal in the cells containing the bound FAM-VAD-FMK can be evaluated with a fluorescence plate reader, fluorescence microscopy, or flow cytometry. The SensoLyte® Caspase Assay Kits are one-step homogeneous assays. Designed in a convenient mix-and-read format, and containing highly sensitive fluorimetric components, SensoLyte® Caspase Assay Kits are an ideal solution for high-throughput screening.
References:
1. Thornberry, NA. and Y. Lazebnik, Science 281, 1312-1316 (1998).
2. Reed, JC. J. Clin. Oncol. 17, 2941-2953 (1999).
3. Lazebnik, YA. et al. Nature 371, 346-347 (1994).
4. Villa, P. et al. Trends Biochem. Sci. 22, 388-393 (1997).
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