San Jose, CA – May 28, 2009 AnaSpec has announced the launch of the industry’s first FRET-based thrombin assay – the SensoLyte? 520 Thrombin Activity Assay Kit.
Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation.1 Thrombin also influences a number of normal and pathological processes, including inflammation,2 tissue repair,3 embryogenesis,4 angiogenesis,5 and tumor invasion.6
The SensoLyte? 520 Thrombin Activity Assay Kit kit employs a novel internally quenched 5-FAM/QXL? 520 FRET substrate (Figure 1). Thrombin cleaves the FRET substrate into two separate fragments resulting in release of the fluorescence of 5-FAM, a green dye. Increase of fluorescence can be monitored at the commonly used excitation and emission wavelengths of 490 nm and 520 nm, respectively. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds, resulting in a high signal to background ratio. Figure 2 shows the inhibition data.
About AnaSpec
AnaSpec is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and combinatorial chemistry.
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