Fluorescent dye-labeled oligos are widely used as primers for sequencing and PCR reactions, and also as probes for fluorescence in situ hybridization (FISH). Double-labeled oligos are used to produce fluorescence resonance energy transfer (FRET) for a number of applications such as real-time PCR assays and automated DNA sequencing. Synthetic oligos can be modified with amine or thiol groups at their 5’-end, 3’-end or internally. These amine and thiol incorporated oligos can then be labeled with amine and thiol reactive fluorophores, respectively.
N-hydroxysuccinimidyl esters (SE) and isothiocyanates of fluorescein and rhodamine derivatives are used for labeling amine-modified oligos. Maleimides and iodoacetamides of fluorescein and rhodamine derivatives are used for labeling thiol-modified oligos.
AnaSpec’s proprietary HiLyte Fluor™ dyes are available in the SE and maleimide forms. These dyes have fluorescence emission wavelengths spanning the full visible and near infrared (NIR) spectrum. HiLyte Fluor™ dyes have properties that are superior to conventional dyes, with high fluorescence quantum yields, large extinction coefficients, good photostability, low pH sensitivity and improved water solubility.
AnaSpec’s HiLyte Fluor™ dyes and classic dyes are excellent choices for labeling amine- and thiol-modified oligonucleotides.
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AnaSpec, EGT Group
AnaSpec, EGT Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and qPCRs. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.
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