Glutathione is one of the most important antioxidant molecules produced by the body. With roles extending from growth and repair to molecular detoxification and excretion, it is ubiquitous in all cells whether eukaryotic or prokaryotic. Although GSH is made constitutively within eukaryotic cells, bacteria are auxotrophic for GSH and need to acquire this molecule by import mechanisms. Bacteria which are unable to import GSH are more susceptible to oxidative and disulphide stress, reducing their pathogenicity and ability to colonise certain host niches within the body. An understanding of such bacterial import proteins may therefore be useful for understanding mechanisms of pathogenicity and infection. This application note describes how the mosquito Crystal was used to dispense 100 nL crystallisation droplets (50 nL protein sample and 50 nL crystallisation condition). This allowed rapid and efficient screening of almost 2000 crystallisation conditions, ultimately resulting in the successful crystallisation and identification of a novel GSH import protein. To read more about this Application please visit: http://www.ttplabtech.com/products/mosquito/bibliography.html. To learn more about the mosquito® Crystal nanolitre pipettor, please visit: http://www.ttplabtech.com/products/mosquito/index.html.
About TTP LabTech Ltd
Based near Cambridge, UK, TTP LabTech supplies laboratory instrumentation for the healthcare, biotech and pharmaceutical sectors. TTP LabTech’s products are designed to increase efficiency, effectiveness, flexibility or overall capability in key biopharmaceutical research processes including compound storage (comPOUND®), low volume liquid handling (mosquito®), high content screening (Acumen®), antibody discovery (Mirrorball®) and sample analysis management (LAB2LAB).
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