Bridging the Gap between Analytical and Process Protein A Chromatography
King of Prussia, PA - Tosoh Bioscience LLC, a provider of chromatographic solutions for the separation of biomolecules, today announced the introduction of the TSKgel Protein A-5PW column. This column is specifically designed for the rapid separation and robust monoclonal antibody titer quantification during cell line selection or upstream bioprocess optimization and control. The TSKgel Protein A-5PW column is now available for sale in the Americas from Tosoh Bioscience LLC.
The TSKgel Protein A-5PW column is packed with 20 µm hydroxylated methacrylic polymer beads coupled with a recombinant Protein A ligand and can be used with high flow rates for high throughput analysis while still maintaining chromatographic efficiency, peak width and resolution. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes. Additionally, the TSKgel Protein A-5PW column demonstrates exceptional column lifetime by allowing more than 2,000 injections without deterioration. The low level of protein A leaching makes this column a good candidate for small scale purification of mAbs for initial characterization.
“We at Tosoh Bioscience are very pleased to introduce the TSKgel Protein A-5PW column to our customers working in monoclonal antibody development,” says Cara Tomasek, Leader of the Product Management Group. “Because of the high flow rate tolerance and durability of the TSKgel Protein A-5PW column, high throughput analysis can be accomplished – an important attribute since there are many harvest cell culture samples that must be screened for IgG titer in early monoclonal antibody development.”
TOSOH BIOSCIENCE LLC
Tosoh Bioscience LLC provides liquid chromatography products to laboratories and manufacturing plants in the Americas. While the majority of our customers work in biotechnology and pharmaceutical companies, our HPLC columns and systems are essential tools for sample analysis in most industries. Our all-in-one dedicated GPC system, the EcoSEC GPC System, is predominantly used in the chemical and polymer industries while our EcoSEC High Temperature GPC System is ideal for the polyolefin and oil industries. In the field of biotechnology, our preparative chromatography resins play a critical role in the purification of proteins and nucleic acids for medical purposes.
The company is a subsidiary of Tosoh Corporation in Japan, the parent of the Tosoh Group, which comprises 141 companies worldwide and a multiethnic workforce of over 10,000 people and generates sales of ¥827 billion (US $8.3 billion) annually.
King of Prussia, PA - Tosoh Bioscience LLC, a provider of chromatographic solutions for the separation of biomolecules, today announced the introduction of the TSKgel Protein A-5PW column. This column is specifically designed for the rapid separation and robust monoclonal antibody titer quantification during cell line selection or upstream bioprocess optimization and control. The TSKgel Protein A-5PW column is now available for sale in the Americas from Tosoh Bioscience LLC.
The TSKgel Protein A-5PW column is packed with 20 µm hydroxylated methacrylic polymer beads coupled with a recombinant Protein A ligand and can be used with high flow rates for high throughput analysis while still maintaining chromatographic efficiency, peak width and resolution. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes. Additionally, the TSKgel Protein A-5PW column demonstrates exceptional column lifetime by allowing more than 2,000 injections without deterioration. The low level of protein A leaching makes this column a good candidate for small scale purification of mAbs for initial characterization.
“We at Tosoh Bioscience are very pleased to introduce the TSKgel Protein A-5PW column to our customers working in monoclonal antibody development,” says Cara Tomasek, Leader of the Product Management Group. “Because of the high flow rate tolerance and durability of the TSKgel Protein A-5PW column, high throughput analysis can be accomplished – an important attribute since there are many harvest cell culture samples that must be screened for IgG titer in early monoclonal antibody development.”
TOSOH BIOSCIENCE LLC
Tosoh Bioscience LLC provides liquid chromatography products to laboratories and manufacturing plants in the Americas. While the majority of our customers work in biotechnology and pharmaceutical companies, our HPLC columns and systems are essential tools for sample analysis in most industries. Our all-in-one dedicated GPC system, the EcoSEC GPC System, is predominantly used in the chemical and polymer industries while our EcoSEC High Temperature GPC System is ideal for the polyolefin and oil industries. In the field of biotechnology, our preparative chromatography resins play a critical role in the purification of proteins and nucleic acids for medical purposes.
The company is a subsidiary of Tosoh Corporation in Japan, the parent of the Tosoh Group, which comprises 141 companies worldwide and a multiethnic workforce of over 10,000 people and generates sales of ¥827 billion (US $8.3 billion) annually.
