CovCheck™ provides an easy ready to use tool to check and confirm the whole genome amplification (WGA) coverage for Next Generation Sequencing (NGS) downstream workflows.
Unbiased DNA amplification and whole genome coverage is one of the most critical aspects within Next Generation Sequencing workflows. The SYGNIS CovCheck™ kit provides NGS users with a valuable quality control method to determine the quality and integrity of the amplified DNA. It provides users with an accurate view of the genomic coverage of the amplified DNA prior to engaging in further additional costly and time consuming NGS downstream processes such as library preparation and DNA sequencing.
CovCheck™ is an end point PCR based kit in a convenient 96-well plate format. No other product available is able to assess all human chromosomes in a single experiment. The SYGNIS CovCheck™ kit includes 24 different sets of primer pairs each targeting a specific human chromosome and enable the evaluation of the genomic coverage of 4 independent single-cell whole genome amplifications experiments simultaneously.
Due to its unique and proprietary design CovCheck™ shows a strong correlation between the samples amplified and the real coverage obtained through whole genome sequencing. The product brings the NGS analysis to a superior level of accuracy and reproducibility and is therefore poised to take a valued position in NGS workflows.
