With the common efforts of its scientists, Creative Biolabs released two hybrid services.
As we know, an understanding of how proteins interact within the cell is a key part of gene functional analysis and potential drug target discovery. The two-hybrid system is a very powerful molecular genetic tool which investigates protein to protein interactions in vivo. This system can be used to study the interaction between two proteins which are expected to interact and find proteins interacting with a protein you have already bait.
Creative Biolabs provides the services on a fee-for-service basis. It is specialized in using Gal4 and LexA based Y2H systems. It is able to perform yeast 2 hybrid screening against integral membrane proteins and membrane-associated/proximal proteins utilizing a modified split-ubiquitin membrane yeast two-hybrid (MYTH) system.
“The advantages and key features of our yeast two-hybrid service include lower rate of false positives, high transformation efficiency, step by step cost-effectiveness, and of course, we are proud to have such experienced technical staff with us”, said Monika Müller, scientific officer of Creative Biolabs.
Yeast One-Hybrid Screening
One-hybrid screening in yeast is a powerful method to rapidly identify heterologous transcription factors that can interact with a specific regulatory DNA sequence of interest. In this technique, the interaction between two proteins (bait and prey) is detected via in vivo reconstitution of a transcriptional activator that turns on expression of a reporter gene.
Detection is based on the interaction of a transcription factor (prey) with a bait DNA sequence upstream of a reporter gene. To ensure that DNA binding results in reporter-gene activation, cDNA expression libraries are used to produce hybrids between the prey and a strong trans-activating domain. Compared to biochemical techniques, the advantage of cloning transcription factors or other DNA-binding proteins via one-hybrid screenings is that the procedure does not require specific optimization of in vitro conditions. Its combination of the 1-hybrid and 2-hybrid approaches increases the stringency and reliability of results obtained from 2-hybrid assays alone.
Creative Biolabs’ combination of the 1-hybrid and 2-hybrid approaches has increased the stringency and reliability of results obtained from 2-hybrid assays alone.
About Creative Biolabs
Creative Biolabs is specialized in providing custom biotechnology and pharmaceutical services that cover the full scope of biotechnology needs of early drug discovery and development, antibody discovery in particular. As a trusted provider of the most cost-effective outsourcing solutions, Creative Biolabs has been working for a large number of satisfied clients from biotechnology and pharmaceutical companies as well as government and academic research laboratories all over the world.
Contact
Bella Smith
Marketing Specialist
1-631-830-6441
marketing@creative-biolabs.com
