SHEFFIELD, UK, 4TH FEBRUARY, 2014: Phase Focus Ltd (Phasefocus), the company that is revolutionising microscopy and imaging with the Phasefocus Virtual Lens®, announces the availability of the Virtual Lens series of label-free live cell imaging systems.
Phase Focus Ltd (Phasefocus) announces a family of innovative phase microscopy systems for live cell imaging using the Phasefocus Virtual Lens®. The Virtual Lens, VL, acquisition and processing engine uses a technique known as ptychography, to provide quantitative, high contrast, label-free images of cells for long term time-lapse studies.
The use of fluorescent probes is commonplace for labelling fixed specimens. However, when used in living cells, these can be toxic and ultimately perturb the natural cell function. The use of laser illumination can also be phototoxic to cells. The Phasefocus VL20 upright and VL21 inverted live cell imaging systems use very low power laser illumination thereby enabling long term time-lapse cell imaging, including multi-area time-lapse, to produce high contrast quantitative image information.
In addition to this, the quantitative nature of the phase information means that data such as cell thickness, cell volume, and cell motility can be measured on a familiar and easy-to-use system for the study of both individual cells and cell populations.
The Virtual Lens is based on technology developed at the University of Sheffield by Professor John Rodenburg. It provides an iterative solution of the diffraction pattern phase problem and is applicable to visible light imaging, X-ray imaging and atomic resolution electron imaging. Phasefocus has applied the technology to develop and install two systems at the University of York in the Department of Biology as one of many technology-leading techniques in the Imaging and Cytometry group managed by Dr Peter O'Toole.
Dr O'Toole describes ptychography as "one of the most important breakthroughs in imaging. It addresses many of the fundamental problems inherent in current microscopy techniques. Put simply, what you get is more than what you see! Artefacts of sample preparation which may arise from labelling or use of markers are negated. I have been most impressed by Phasefocus and their ability to quickly respond to feedback and then to develop products to meet the needs of the user."
In conjunction with the development of the Virtual Lens, Phasefocus is supporting a KTP Associate at York, - Dr Rakesh Suman - who works in Dr O'Toole's group tutoring members of the department in the use of the technique for the study of many different sample types including cancer and stem cells and applications relating to immunology and neurology.
For more information on ptychography and its application for label-free, live cell imaging, please visit the Phasefocus web site: http://www.phasefocus.com/.
To find out about the company and to learn more about using Phasefocus' unique lens-less solutions for electron microscopy, label-free live cell cycle analysis and metrology, visit http://www.phasefocus.com/.
ABOUT PHASEFOCUS:
Phasefocus is a venture capital-backed spin-out company from the University of Sheffield founded in 2006. Its principal equity investors are Ombu Group and Fusion IP plc. In February 2013, the Company announced that it had entered into a Licencing Agreement with Gatan Inc., the world's leading manufacturer of instrumentation and software used to enhance and extend the operation and performance of electron microscopes. A prototype "add-on" product for existing electron microscopes has already demonstrated an improvement in resolution of a factor of five over that available using the host instrument's conventional electron optics. Its products also include an optical microscope used for label-free time-lapse imaging of biological cells, and an Ophthalmic Lens Profiler that can measure soft contact lenses with unprecedented levels of resolution and accuracy.
Technology reference paper:
http://www.nature.com/srep/2013/130806/srep02369/full/srep02369.html: 1Ptychography - a label free, high-contrast imaging technique for live cells using quantitative phase information: Joanne Marrison, Lotta Räty, Poppy Marriott & Peter O'Toole. Scientific Reports 3, Article number: 2369 doi:10.1038/srep02369.
For further information:
Please contact Phasefocus direct or their marketing agency, Talking Science:
Phase Focus Limited
Electric Works
Sheffield Digital Campus
Sheffield, S1 2BJ, UK
T +44(0)114 286 6377
M +44(0)7985 042392
http://www.phasefocus.com/
ianpykett@phasefocus.com
Talking Science Limited
39 de Bohun Court
Saffron Walden
Essex CB10 2BA UK
T +44(0)1799 521881
M +44(0)7843 012997
www.talking-science.com
jezz@talking-science.com
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