Well-designed qPCR assays require the careful consideration of primer placement, specificity, avoidance of SNPs, oligo interactions and accurate Tm calculations. In order to meet these requirements, IDT now offers PrimeTime Pre-designed qPCR Assays for all genes in the human, mouse and rat genome. These assays avoid any cross-reactivity within that genome, known SNPs and primer interactions, while providing full disclosure of all sequence information, as recommended by MIQE guidelines1. By including the ZEN double-quenched probes, these assays provide outstanding levels of sensitivity, with the added security of knowing precise probe location.
IDT’s ZEN double-quenched probe technology increases the accuracy and reliability of 5’ nuclease qPCR experiments by positioning an internal ZEN quencher 9 bases from the 5’ fluorophore. When combined with the standard 3’ quencher, this significantly decreases background fluorescence and increases sensitivity. The initial background fluorescence signal is therefore much lower, making subsequent changes easily detectable. As a result low background is obtained, even at 40 base pairs or longer.
For more information on IDT’s PrimeTime Pre-designed qPCR assays and the ZEN double-quenched probe technology, please visit booth 16 at qPCR, or www.idtdna.com.
1. The MIQE Guidelines – Minimum information for publication of quantitative real time PCR experiments. Available at: http://www.gene-quantification.de/miqe.html
About IDT
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; and Leuven, Belgium. For more information visit www.idtdna.com.
Integrated DNA Technologies 800-328-2661 (US & Canada) +1 319-626-8400 (outside US) productinfo@idtdna.com www.idtdna.com
