Make It Glow: Intro to Molecular Cloning

ABOUT THE EVENT

Date: December 6, 2025 2pm - December 13, 2025 6pm EST
Event Location: Brooklyn, New York

In this hands-on workshop, you’ll step into the role of a molecular biologist to reprogram living cells using DNA!

In-person 3 part class:

Saturday, December 6th from 2 PM- 6PM EDT

Sunday, December 7th from 2 PM- 6PM EDT

Saturday, December 13th from 2 PM- 6PM EDT

How is it possible to build new forms of life, like bacteria that glow in the dark or yeast that smells like bananas? In this hands-on workshop, you’ll step into the role of a molecular biologist to reprogram living cells using DNA.

You’ll learn how to “cut and paste” genes - the biological code that determines how living things look and behave–and use those skills to make bacteria glow a different color. Starting with cells that produce green fluorescent protein (GFP), you’ll engineer a new version of their DNA that glows red instead by swapping out one gene for another using Gibson Assembly, a powerful cloning method used in research labs around the world.

During the course, you’ll explore how any gene can be switched on and off and discuss how genetic engineering is reshaping everything from medicine to materials to food.

You’ll learn to:

  • Amplify and edit DNA using polymerase chain reaction (PCR)
  • Use restriction enzymes and Gibson Assembly to swap fluorescent genes
  • Transform E. coli with your new plasmid and make them glow
  • Analyze your results under UV light
  • Design your own DNA experiments using Benchling, a professional biotech tool

No prior experience needed - just curiosity and a steady hand.

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