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Research Associate - Conklin Lab

Employer
Gladstone Institutes
Location
San Francisco, CA
Start date
Feb 9, 2024

View more

Discipline
Regulatory, Science/R&D, Research, Laboratory
Required Education
Bachelors Degree
Position Type
Full time
Hotbed
Biotech Bay

Job Details

Category:Science
Lab/Area:Conklin Lab

Description:

Description:

The Conklin Lab is seeking a Research Associate I or II, to support research projects aimed at developing CRISPR therapies for neurodegenerative diseases. Project goals include: using iPSCs and iPSC-derived neurons to test CRISPR therapies in patient-derived cells, conducting CRISPRi screens to discover factors that influence gene editing efficiency, and investigating how neuronal DNA repair affects gene editing outcomes. Ideal candidates have strong communication skills, with a preferred background in mammalian cell culture and molecular biology. Applicants interested in progressing to graduate school or medical school within 2-3 years are encouraged to apply.

Please submit a resume/CV, unofficial transcript, and cover letter describing your interest in the lab.

About the Lab:

In the Conklin Lab, we focus on developing gene editing based therapies to treat neurodegenerative diseases. We leverage our expertise in iPSC-based cell models and CRISPR-Cas9 gene editing, to both model genetic diseases and test the effectiveness of gene editing therapies in patient-derived cells. Using state-of-the-art tools to deliver gene editing proteins into human neurons, we have started to identify some of the key barriers making CRISPR editing so difficult in neurons. Now, we are poised to develop cutting-edge strategies to overcome these barriers, hopefully unlocking the full potential of CRISPR editing in clinically relevant cells.

In addition to these molecular biology techniques, we are also using computational methods to optimize precise genome editing. For example, we are developing high-throughput methods to find combinations of CRISPR-Cas9 reagents that could optimally treat large and diverse patient populations. These efforts are crucial for making gene editing therapies cost-effective and accessible, to benefit as many people as possible.

Duties and Responsibilities:

The Research Associate will be directly supervised by a Graduate Student or Postdoctoral Fellow and have regular meetings with the PI, Dr. Bruce Conklin.

The main responsibilities of this position will include but are not limited to:

  • Human pluripotent stem cell culture, cell line engineering, and differentiation into neurons.

  • Molecular biology assays including PCRs and next-generation sequencing, to measure editing outcomes.

  • Molecular cloning to generate new plasmid constructs for use in gene editing experiments.

  • Production of virus-like particles and lipid nanoparticles to deliver Cas9 or other gene editing proteins to cells.

  • Maintaining a detailed and thorough lab notebook.

  • Contributing to shared common lab duties, such as maintaining stocks, reagents, and equipment.

  • Routinely presenting findings, in the form of lab meeting presentations.

Required Qualifications:

  • BA/BS in molecular biology, cell biology, bioengineering, or a related scientific field.

  • One or more years of experience working/volunteering in a biological research lab.

  • Experience with molecular biology techniques (PCR, molecular cloning, sequencing).

  • Knowledge of scientific equipment (centrifuge, pipettes, thermocyclers, etc.) and basic lab etiquette.

  • Strong communication skills with a history of presenting at scientific group meetings.

  • Attention to detail and meticulous record keeping abilities.

  • Flexibility in schedule; ability to work evenings and weekends when necessary.

  • Familiarity or interest in human genetics/genetic disease and/or CRISPR gene editing.

Preferred Qualifications:

  • Experience with human or mammalian cell culture.

  • Experience with CRISPR gene editing, or CRISPR screening.

  • Experience with fluorescence microscopy, next-generation sequencing, flow cytometry, or other biological readouts.

Hiring Range:

RA I

$25/hr $30/hr


RA II

$66K -$78K


Gladstone Perks & Benefits

  • People–work with talented, committed, and supportive teammates within an organization that values each member of its community.

  • A meaningful place to grow and learn–whether it’s your professional skills or scientific knowledge, we have the resources and environment to advance either so you can better support Gladstone’s mission to drive a new era of discovery in disease-oriented science and to mentor tomorrow’s leaders in an inspiring and diverse environment.

  • Healthy work/life balance–you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work.

  • Compensation–competitive salary. Title and salary will be commensurate with education and experience.

  • Excellent benefits–generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation.

​Gladstone is committed to providing equal employment opportunities to all employees and applicants for employment without regard to race, color, sex, religion, national origin, ancestry, age, marital status, medical condition, physical or mental disability, veteran status, sexual orientation, or any other non-job related characteristic. We make all employment decisions so as to further this principle of equal employment.

Company

Gladstone is an independent, nonprofit life science research organization located in the epicenter of biomedical and technological innovation in the San Francisco Bay Area. Gladstone has created a research model that disrupts how science is done, funds big ideas, and attracts the brightest minds. Gladstone Institutes leads the way for innovative research in the biomedical field. We always seek dedicated and talented individuals to join our team.

Scientists know that using a diversity of approaches is often the most efficient strategy for solving a problem. New and different perspectives engender the very innovation that Gladstone prizes. This is one of the many reasons why we value diversity at Gladstone—be that a diversity of race, culture, religion, mental or physical ability, heritage, age, gender, sexual orientation or other characteristics. Our employees hail from more than 30 countries around the globe and we seek to hire the best and brightest for our diverse team of scientists and administrators. Career opportunities are available in both our administrative offices and our research areas.

Our Benefits
Gladstone offers eligible employees—and their dependents—a comprehensive range of benefits, including health insurance, retirement savings, and professional development.

Highlights

  • Health and Retirement
    • A choice of medical plans, flexible spending account, and retirement account options that include employer contributions help you take care of your health and your future.
  • Time Away from Work
    • More than just vacation days, our paid and unpaid leave policies offer flexibility to help you confront different stages of life including maternity and paternity leave, as well as other types of family leave.
  • Professional Development
    • Gladstone encourages employees to seek the skills they need to remain at the forefront of their profession. By supporting professional development workshops and continuing education, Gladstone provides employees with the resources they need to evolve in their role and in their career.
  • Gladstone Cares
    • A specially selected suite of benefits to support you in and out of the workplace. Includes resources for dealing with the many stages of life to specific coverage for family planning, and back-up child or elder care coverage.
Company info
Website
Location
1650 Owens Street
San Francisco
California
94158
United States

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