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Research Associate - Preclinical Team (In vitro Technician)

Employer
Gladstone Institutes
Location
San Francisco, CA
Start date
Nov 22, 2023

View more

Discipline
Regulatory, Research/Documentation, Science/R&D, Research
Required Education
Bachelors Degree
Position Type
Full time
Hotbed
Biotech Bay

Job Details

Category:Science
Lab/Area:GIGI Central Science

Description:

The Gladstone-UCSF Institute of Genomic Immunology is recruiting a Research Associate (RA) to help perform in vitro experiments related to genetic engineering and functional assessment of T cell therapies, primarily CAR-T cells. Our institute has pioneered the application of novel genetic engineering technologies such as CRISPR to primary human immune cells. These new technical abilities have furthered our research into the genetic control of T cell biology as well as opened the door to novel cancer immunotherapies based on engineered human immune cells. This individual would be part of a preclinical research team under the direction of Justin Eyquem (UCSF) streamlining the generation, benchmarking, and functional assessment of genetically engineered human T cell therapies for cancer and inflammatory disease.

Key Responsibilities

The RA will utilize various protocols to generate the molecular components, such as DNA constructs, gRNA library, lentiviral vectors and Adeno-Associated Vectors necessary for specific cellular engineering experiments. The technician’s role will also include the design and completion of cellular editing experiments, as well as in vitro analysis of novel cellular cancer immunotherapies and coordination with the in vivo team for mouse experiments employing the T cell products under development. Additional responsibilities will consist of isolation and culture of primary human T cells, as well as functional analysis of engineered cells via Sanger sequencing, next-generation DNA sequencing, Single-Cell RNA sequencing, and flow cytometry. Strong organizational and communication skills are required.

  • Preparation of NGS library

  • Design and cloning of CRISPR library

  • Production of lentiviral vectors for gRNA library and transduction of primary cells

  • Primer design and PCR troubleshooting

  • Assist with cell culture and preparation for subQ injections or IV injections

  • Assist with dissection of the spleen, lymph nodes, and engrafted tumor for downstream analysis

  • Work collaboratively across a dynamic scientific environment

  • Maintain and ensure a safe laboratory environment and work practices. Follow standard operating procedures (SOP) and IACUC protocols.

  • Other related duties as assigned

Required Qualifications:

  • AA or AS and 2+years related experience or BS or BA or MS in Molecular Biology, Chemistry Biochemistry, Microbiology, or related field; or an equivalent combination of education and experience

  • New graduates with a commitment to learning are welcome to apply

  • Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.)

  • Excellent attention to detail, organizational and interpersonal communication skills (verbal and written)

  • Willingness and ability to learn new methods and skills for changing research priorities

  • Ability to work independently and as a member of a research team

  • Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines

  • Ability to comply with policies and procedures

  • Some evening and weekend hours are required

  • Design and cloning of CRISPR library

  • Production of Lentiviral vector for gRNA library and transduction of cell lines or primary cells

Preferred Qualifications

  • One or more years of laboratory experience utilizing techniques or methods required by the position

  • Prior experience with DNA sequencing technologies, including Sanger and NGS

  • Prior experience in the design and assembly of DNA constructs (via restriction digestion and ligation, Gibson assemblies, Golden-Gate assemblies, etc.)

  • Prior experience with mammalian cell culture, especially T cell culture

The Gladstone-UCSF Institute of Genomic Immunology is committed to improving diversity, equity, and inclusion in the genomic immunology field, from trainees to faculty, and is an equal- opportunity employer.

Hiring Range:

Research Associate I- $25/hr-$29/hr

Research Associate II- $65K-$75K


Gladstone Perks & Benefits

  • People–work with talented, committed, and supportive teammates within an organization that values each member of its community.

  • A meaningful place to grow and learn–whether it’s your professional skills or scientific knowledge, we have the resources and environment to advance either so you can better support Gladstone’s mission to drive a new era of discovery in disease-oriented science and to mentor tomorrow’s leaders in an inspiring and diverse environment.

  • Healthy work/life balance–you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work.

  • Compensation–competitive salary. Title and salary will be commensurate with education and experience.

  • Excellent benefits–generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation.

​Gladstone is committed to providing equal employment opportunities to all employees and applicants for employment without regard to race, color, sex, religion, national origin, ancestry, age, marital status, medical condition, physical or mental disability, veteran status, sexual orientation, or any other non-job related characteristic. We make all employment decisions so as to further this principle of equal employment.

Company

Gladstone is an independent, nonprofit life science research organization located in the epicenter of biomedical and technological innovation in the San Francisco Bay Area. Gladstone has created a research model that disrupts how science is done, funds big ideas, and attracts the brightest minds. Gladstone Institutes leads the way for innovative research in the biomedical field. We always seek dedicated and talented individuals to join our team.

Scientists know that using a diversity of approaches is often the most efficient strategy for solving a problem. New and different perspectives engender the very innovation that Gladstone prizes. This is one of the many reasons why we value diversity at Gladstone—be that a diversity of race, culture, religion, mental or physical ability, heritage, age, gender, sexual orientation or other characteristics. Our employees hail from more than 30 countries around the globe and we seek to hire the best and brightest for our diverse team of scientists and administrators. Career opportunities are available in both our administrative offices and our research areas.

Our Benefits
Gladstone offers eligible employees—and their dependents—a comprehensive range of benefits, including health insurance, retirement savings, and professional development.

Highlights

  • Health and Retirement
    • A choice of medical plans, flexible spending account, and retirement account options that include employer contributions help you take care of your health and your future.
  • Time Away from Work
    • More than just vacation days, our paid and unpaid leave policies offer flexibility to help you confront different stages of life including maternity and paternity leave, as well as other types of family leave.
  • Professional Development
    • Gladstone encourages employees to seek the skills they need to remain at the forefront of their profession. By supporting professional development workshops and continuing education, Gladstone provides employees with the resources they need to evolve in their role and in their career.
  • Gladstone Cares
    • A specially selected suite of benefits to support you in and out of the workplace. Includes resources for dealing with the many stages of life to specific coverage for family planning, and back-up child or elder care coverage.
Company info
Website
Location
1650 Owens Street
San Francisco
California
94158
United States

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