Principal Research Associate, Gene Editing, Lentiviral Screening

The Role

As part of our vision, Moderna is committed to invest in R&D to further expand our impact on patients. We are building an independent research and business unit for genomic medicines, called Moderna Genomics (mGx) based in Technology Square, Cambridge, MA. mGx, striving to be an industry leader in genomic medicines, will be an innovation engine for the company focused on discovering and delivering the next generation of in vivo gene editing and nonviral gene therapies.

We are seeking a highly skilled and motivated cell biologist for a position at Moderna Genomics in Cambridge, MA. The individual will be responsible for the development and execution of lentivirus-based high-throughput pooled assays in support of Moderna Genomics discovery programs. The ideal candidate will play an integral role in advancing our gene editing platform utilizing a wide range of molecular and cellular biology techniques. Extensive experience in lentivirus transduction, mammalian cell culture, transfection, flow cytometry and cell-based assays such as Western blot/JESS, ELISA, and Luminex is required.

Applicants should be team players, able to thrive in a highly collaborative and fast-paced environment.

Heres What Youll Do

• Generate stable cell lines through transduction of lentiviral vectors, antibiotic selection, and flow cytometry. Establish and maintain Cell Bank to support Moderna Genomics programs.

• Engineer mammalian cells by introducing plasmid DNA, mRNA, and gRNA by appropriate methods, including lipid-based transfection and electroporation. Implement molecular biology techniques including nucleic acid extraction and PCR.

• Perform large-scale cell culture of adherent and suspension mammalian cell lines and primary cells to support high-throughput screens.

• Develop and execute screening assays to quantify in vitro biomarker expression, including protein expression, cytokine expression, and reporter assays. Establish quantitative methods (e.g. ELISA, MSD, ELLA, Western Blot/JESS, qPCR, Luminex) as needed.

• Routinely generate, analyze, interpret, and present high quality experimental data in accordance with project timelines. Accountable for project success and results delivery.

• Maintain an accurate and detailed record of experiments. Execute and troubleshoot protocols, independently adapt new protocols into practice, and query the literature to incorporate additional assays as needed.

• Communicate research and development findings with internal and external partners by written and oral presentations.

Heres What Youll Need (Minimum Qualifications)

• BS/BA with minimum 5 years experience or MS with minimum 3 years of industry experience in Cell Biology, Biochemistry, or related science.

• Demonstrated expertise in mammalian cell culture, cell-based assays and basic molecular biology techniques is required.

• Experience developing and performing cell-based and bioanalytical assays (Western blot/JESS, colorimetic or luminescent reporter assays, ELISA/Ella, MSD, Luminex, etc.) is required.

Heres What Youll Bring to the Table (Preferred Qualifications)

• Experience designing, executing and analyzing experiments independently. Ability to independently troubleshoot and correct technical issues.

• Excellent interpersonal, organizational, and collaborative skills, attentive to detail, and the ability to work independently and effectively in a dynamic environment.

• Ability to multi-task to meet aggressive timelines and development goals.

• Experience with fluorescence imaging, including live-cell and fixed confocal imaging, Opera Phenix and Harmony experience would be a bonus.

• A desire to make an impact as part of a high-growth, transformational company that is Bold, Relentless, Curious, and Collaborative.

Moderna is pleased to provide you and your family with a comprehensive and innovative suite of benefits, including:

• Highly competitive and inclusive medical, dental and vision coverage options
• Flexible Spending Accounts for medical expenses and dependent care expenses
• Lifestyle Spending Account funds to help you engage in personal enrichment and self-care activities
• Family care benefits, including subsidized back-up care options and on-demand tutoring
• Free premium access to fitness, nutrition, and mindfulness classes
• Exclusive preferred pricing on Peloton fitness equipment
• Adoption and family-planning benefits
• Dedicated care coordination support for our LGBTQ+ community
• Generous paid time off, including:
  Vacation, sick time and holidays
  Volunteer time to participate within your community
  Discretionary year-end shutdown
  Paid sabbatical after 5 years; every 3 years thereafter
• Generous Paid Leave offerings, including 18 weeks of 100% paid parental leave for all new parents
• 401k match and Financial Planning tools
• Moderna-paid Life, LTD and STD insurance coverages, as well as voluntary benefit options
• Complimentary concierge service including home services research, travel booking, and entertainment requests
• Free parking or subsidized commuter passes
• Location-specific perks and extras!

About Moderna

Since our founding in 2010, we have aspired to build the leading mRNA technology platform, theinfrastructure to reimagine how medicines are created and delivered, and a world-class team. We believe in giving our people a platform to change medicine and an opportunity to change the world.

By living our mission, values, and mindsets every day, our peopleare the driving force behind our scientific progress and our culture.Together, we are creating a culture of belonging and building an organization that cares deeply for our patients, our employees, the environment, and our communities.

We are proud to have been recognized as a Science Magazine Top Biopharma Employer, a Fast Company Best Workplace for Innovators, and a Great Place to Work in the U.S.

If you want to make a difference and join a team that is changing the future of medicine, we invite you to visit modernatx.com/careers to learn more about our current opportunities.

Moderna is a smoke-free, alcohol-free and drug-free work environment.

Moderna is a place where everyone can grow. If you meet the Basic Qualifications for the role and you would be excited to contribute to our mission every day, please apply!

Moderna is proud to be an equal opportunity workplace and is an affirmative action employer.

Moderna is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person's race, color, sex, gender identity or expression, age, religion, national origin, ancestry or citizenship, ethnicity, disability, military or protected veteran status, genetic information, sexual orientation, marital or familial status, or any other personal characteristic protected under applicable law. Moderna is an E-Verify Employer in the United States. We also consider qualified applicants regardless of criminal histories, consistent with legal requirements.

Belonging, Inclusion, and Diversity are critical to the success of our company and our impact on society. Were focused on attracting, retaining, developing, and advancing our employees and believe that by cultivating diverse experiences, backgrounds, and ideas, we can provide an environment where every employee is able to contribute their best.

Moderna is committed to offering reasonable accommodations to qualified job applicants with disabilities. Any applicant requiring an accommodation in connection with the hiring process and/or to perform the essential functions of the position for which the applicant has applied should make a request to the Talent Acquisition Partner or contact the Accommodations team at leavesandaccommodations@modernatx.com. (EEO/AAP Employer)

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