Scientist I - Cytokines and HIV

Scientist I - Cytokines and HIV

Job ID: req3454
Employee Type: exempt full-time
Division: Applied & Development Research Program
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA

The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc.  The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.

Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.

PROGRAM DESCRIPTION

Laboratory of Human Retrovirology and Immunoinformatics (LHRI), Applied/ Developmental Research Directorate, has investigated the pathogenesis of virus infection and innate immune responses against infected viruses (HIV, HSV, and KSHV) using immunological, microbiological, biological, and bioinformatics techniques in Basic and Translational/Clinical Research (https://david.ncifcrf.gov/). Our research aims to develop novel immune therapies to support NIAID clinical studies for patients infected with multiple-class drug-resistant (MDR) HIV or HIV-related infectious diseases.

The Basic research section of LHRI has discovered Interleukin-27 (IL-27) as a novel anti-virus cytokine, IL-27-induced novel anti-micro RNAs, and novel innate immune response against virus infection in human immune system. The section seeks a productive and creative scientist. 

KEY ROLES/RESPONSIBILITIES

• Investigation of the function of cytokines (including IL-27) on HIV replication in Myeloid immune cells populations
• Characterization of novel subsets of virus-resisting macrophages, dendritic cells and microglia
• Present in scientific conferences
• Publish research articles as the first authors and co-authors

**THIS POSITION IS CONTINGENT UPON FUNDING APPROVAL** 

BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

• Possession of a PhD degree from an accredited college or university according to the Council for Higher Education Accreditation (CHEA). (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency
• No experience required beyond education requirement
• Primary cell culture and fundamental molecular biology techniques
• Gene delivery using lentivirus transduction
• qRT-PCR
• Western Blotting
• Multiplex or single ELISA
• Construction of expression plasmids encoding the gene of interest 
• Ability to obtain and maintain a security clearance

PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration:  

• More than two (2) years of experiences in a single lab with publication(s) or in press manuscript(s) with the first authorship(s)
• Knowledge in biological functions of cytokines and signal transduction
• Handling infectious viruses (HIV or other viruses in BSL2* or BSL3 lab)
• Digital qPCR
• Manipulation of confocal microscope
• Isolation of virus particles using ultracentrifugation
• Protein isolation
• Illumine next-generation sequence, 10x genomic chromium controller
• WB/FISH/IHC,  FPLC/protein isolation

JOB HAZARDS

• This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunization 

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)

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