Sr. Research Associate I, Flow Cytometry

Job Type Full-time

Position Summary

The Sr. Research Associate I will independently design and plan flow cytometry experiments, with supervisor review, to meet project goals related to the discovery and development of therapeutic antibodies. This role will require the ability of think critically and refine experimental methods, including review of relevant scientific literature on technical aspects of the job requirements. This role will also require knowledge of flow cytometry data analysis and presentation, as well as the sharing of experimental findings in team settings. This position works in a matric environment with cell biologists, translational medicine scientists, molecular biologists and protein scientists, offering the opportunity to perform complex FACS based experiments to support projects during the antibody characterization and preclinical development phase of antibody development. A strong background in the theories and principles of flow cytometry is required.

Essential Functions

• Perform flow cytometry based experiments in support of internal projects
• Characterization of proteins of interest on primary cells 
• Experience with sterile technique and mammalian cell culture, including both established cell lines and culture of primary immune cells
• Knowledge of functional assays for both B and T cells. 
• Development of cell based FACS assays, including, but not limited to, cell proliferation, cell signaling, intracellular staining and receptor occupancy
• Expertise with flow cytometry, including analysis of ligand/receptor binding, receptor quantification, and intracellular cytokine detection
• Experience with high content multi-color flow cytometry experiments and instrumentation (>13 colors).
• Familiarity with analysis of scientific datasets, including FlowJo, Excel and GraphPad PRISM
• Presentation of experimental results at project & department meetings
• Assists more junior associates with troubleshooting
• Stock and maintain flow cytometry facility

 

Requirements

Education & Experience: 

• Bachelors degree required in biology, biochemistry or related field
• 4 – 8 years relevant laboratory experience with 2-4 years flow cytometry experience required, cell sorting experience a plus
• Understanding of and background in Cellular Biology.
• (FACS staining, Assay Development/Optimization)

Knowledge and Competencies: 

• Knowledge of the theories and principles of flow cytometry and the application of those practices in flow cytometry
• Proficiency in working with one or more FACS analysis software(s) (i.e. Spigot, Sortware, FlowJo, FACSDiva, CellQuest Pro, NovoExpress)
• Ability to work with complex instrumentation and to manipulate them to achieve optimum performance
• Demonstrated ability to perform laboratory tasks safely, rapidly, accurately, and efficiently
• Ability to communicate clearly, and to report and present experimental results and analysis to department colleagues
• Ability to work effectively in as a team player in a complex, fast-paced entrepreneurial environment
• Self-motivated, independent, and results-oriented
• Capacity to organize assignments, work within deadlines
• Position may require occasional evening and/or weekend commitment
• Position may require working with biological and/or chemical hazards
• Position might require lifting up to 20 pounds

  

Physical Demands

The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

While performing the duties of this job, the employee is regularly required to sit and talk or hear. The employee frequently is required to use hands to finger, handle, or feel. The employee is occasionally required to stand, walk, and reach with hands and arms. The employee must occasionally lift and/or move up to 10 pounds. Specific vision abilities required by this job include close vision and ability to adjust focus in order to read.

Work Environment

The work environment characteristics are representative of those an employee encounters while performing the essential functions of this job, typically in an open office environment. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

This position requires working with biological and/or chemical hazards

All qualified applicants will receive consideration for employment without regard to race, color, sex, sexual orientation, gender identity, religion, national origin, disability, veteran status, age, marital status, pregnancy, genetic information, or other legally protected status. 

Salary Description $82,600-$106,400, Stock Options & RSUs, 10% 401k