Research Technician

Research Technician

Job ID: req2821
Employee Type: nonexempt full-time
Division: Applied & Development Research Program
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA

The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc.  The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.

Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.

PROGRAM DESCRIPTION
The In Vivo Evaluation Group, BTB, DTP is establishing both in vitro and in vivo tumor lines from patient biopsies from DCTD sponsored clinical trials with the goal of developing models for NCI’s Patient Derived Models Repository (PDMR). The PDMR is a repository of patient-derived models that include in vivo tumor fragments, in vitro tumor lines (2D and 3D), cancer associated fibroblasts, and DNA/RNA isolates.  The goal of the PDMR is to distribute well validated and highly characterized material to researchers worldwide. A secondary mission is to evaluate the efficacy of approved oncology drugs in combination or as single agents in a subset of rare and recalcitrant PDX tumor models.

KEY ROLES/RESPONSIBILITIES

• Establish 2D cell cultures from patient samples and from tumors isolated from mice implanted with human tumors
• Aid in the daily maintenance, passaging and cryopreservation of the various 2D & 3D cultures grown in different medias
• Perform routine cell culture tasks including cell line maintenance, preparation of large batches of cells (25-150 flask harvests) for drug studies or cryopreservation, growth of cells on a variety of supports.
• Prepare all required medias, components and reagents used in the culture of 2D and 3D cell cultures
• Participate in the maintenance of proper records is critical as each patient derived cell mixture/clone/line will be passaged and expanded multiple times with the intent of delivering carefully documented samples to researchers worldwide.
• Aid in other routine tasks as needed such as cell photography, tube/plate labeling, cell deliveries/pick-ups etc
  
  BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below: 

• Possession of a BS/BA degree from an accredited college or university (according to CHEA). (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency.
• Ability to be flexible to meet changing needs
• Organizational skills and database management skills
• Ability to work in a fast paced environment
• Ability to work under Biosafety Level-2 conditions
• Ability to obtain and maintain a security clearance 
• Knowledge of Microsoft office programs (Word, Excel, PowerPoint)
• Organizational skills and database management skills
• Ability to maintain precise records
• Good written and verbal communication skills and attention to detail
  
  PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration:  

•  Knowledge of sterile eukaryotic tissue culture techniques
• Knowledge of organoid cell culture
  
  JOB HAZARDS

• Job hazard or requirement  Enroll in OSHA BBP.
• Must be medically cleared to receive any necessary immunizations per protocol requirements.
• Job hazard or requirement  Employee would receive vaccinations required and determined by Occupational Health Services (OHS) and/or mandated by Federal Health Standard Regulations
  Job hazard or requirement
• This position is subject to working with or have potential for exposure to animal(s)
  and/or animal material(s)
• This position is subject to working with or have potential for exposure to infectious
  material, requiring medical clearance and immunizations

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)

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