Laboratory Technician

We are currently searching for a Laboratory Technician to support the research activities of the NINDS Viral Production Core Facility. This is a full-time, on-site opportunity in Bethesda, MD.  

• Perform a variety of laboratory techniques and procedures.
• Work with a variety of routine and specialized laboratory instruments and equipment in the laboratory.
• Collect data and prepare for analysis and interpretation.
• Perform fundamental procedures required for research projects.
• Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis.
• Follow established procedures and protocols; utilize aseptic technique to perform experiments.
• Perform routine analysis and tests on specimens in the laboratory.
• Perform experiments on samples according to protocols.
• Perform routine experiments and tests; follow established procedures and protocols.
• Prepare materials and equipment for experiments.
• Maintain stocked supplies and records of working reagents, including fixatives, buffers, solvents, and staining.
• Process and organize multiple samples simultaneously.
• Ensure that all specimens and equipment necessary for experiments are available and in good order.
• Prepare specimens and equipment for use in the laboratory.
• Maintain inventory system and sample storage according to outlined guidelines.
• Ensure that policies and procedures regarding sample retrieval and use are followed.
• Work with staff to implement storage and distribution of biological samples for future scientific research.
• Update and maintain written laboratory record of the work.
• Collect and enter research data into laboratory notebooks and databases.
• Ensure that supplies and consumables are ready and on hand as necessary for maximum efficiency in the laboratory.
• Ensure that adequate clean inventory is available for immediate usage.
• Perform fundamental procedures required for research projects.
• Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis.
• Monitor and inventory routine laboratory supplies; ensure that supplies are stocked and organized.
• Plan and organize requests for basic and extraordinary laboratory needs for supplies and consumables.
• Ensure that supplies and consumables are ready and on hand as necessary for maximum efficiency in the laboratory.
• Monitor inventory levels and proper storage of materials and supplies in accordance with established policies and procedures.
• Establish and maintain a record of laboratory supply usage.
• Inventory laboratory supplies and equipment, make recommendations and prepare purchase requests.
• Work with staff to maintain stocked supplies and records of working reagents, including fixatives, buffers, solvents, and staining.
• Ensure that reagents are made correctly and that expired reagents or defective products are replaced to ensure validity of results.
• Order and maintain laboratory supplies, reagents, and equipment.
• Perform laboratory maintenance tasks, including scheduling liquid waste container pickup.
• Monitor equipment performance and maintenance schedules.
• Assist in troubleshooting and calibrating various laboratory equipment and instruments.
• Coordinate general laboratory maintenance, including equipment upkeep and calibration.
• Perform calibration, run controls, and enter results for various equipment.
• Carry out calibration; organize and initiate service and preventative maintenance on equipment.
• Monitor and maintain contracts for laboratory equipment and monitor equipment performance and maintenance schedule.
• Perform fundamental procedures required for research projects.
• Provide experimental progress updates on procedures performed in the laboratory.
• Record details of experimental procedures, results, and protocols.
• Perform laboratory maintenance tasks, including scheduling liquid waste container pickup.
• Provide technical help and expertise to lab members.
• Prepare training materials and presentations for staff training.
• Provide one-on-one and group training related to safety standards on usage and disposal of chemicals and reagents.
• Provide training to staff on the performance of laboratory experiments, assays, and protocols.
• Train new laboratory members in the correct use of equipment, application of techniques and laboratory protocols.
• Engage in problem solving as required to solve technical difficulties and to ensure steady progress, with a goal of providing high quality experimental data suitable for publication.
• Troubleshoot issues with technical procedures; utilize trial and error methods customize protocols to the biological systems utilized in the laboratory.

#SOAR

• Bachelor’s degree in Molecular Biology
• At least 1 years of experience
• Good Laboratory Practice (GLP)
• Produce purified subclinical grade AAV and lentiviruses by completing a variety of cellular and molecular methodologies and assays including Plasmid DNA cloning; Tissue culture; FLPC; Ultracentrifugation; RNA Isolation; Real-time PCR (RTPCR)
• Perform experiments in accordance with protocols
• Provide general lab management including stocking of supplies and reagents
• Operate and routinely maintain and calibrate laboratory equipment
• Data Analysis
• Maintain a clean and organized laboratory working environment
• Procure laboratory supplies, reagents, and equipment
• Comply with laboratory safety procedures and regulatory policies to ensure a safe laboratory environment
• Maintain a daily notebook that includes accurate, detailed records of laboratory experiments
• Pipetting
• Polymerase chain reaction (PCR), qPCR, RT-PCR, RT-qPCR
• Molecular Cloning
• Gel Electrophoresis
• Nucleic Acid Extraction
• SOP writing
• Work under BSL 1 and BSL 2 level
• Equipment Maintenance
• Inventory Management
• Ordering Supplies
• Maintaining Stock Solutions
• DNA Purification
• RNA purification
• Nucleic acid quantification
• Cell culture
• NIH POTS
• Outlook

Dovel Technologies and its Family of Companies (Medical Science & Computing and Ace Info Solutions) was acquired in October 2021.

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